Abstract

We have devised a new method to measure cytotoxic T cell activity in vitro and perfected a method to measure immune rejection in vivo. Using these techniques, in combination with our standard thymic organ culture, we are in the process of studying four questions. 1.Do newly born T cells leave the thymus in a tolerizable or activateable state. 2.Does thymic epithelium present antigens in a tolerogenic or immunogenic fashion. 3.Does T cell priming and/or memory require the presence of B cells. 4.What is the frequency of alloreactive T cells. Our preliminary experiments suggest that newly developed CD4+ and CD8+ T cells are fully capable of responding to allogeneic stimulators, thus refuting models that require the assumption that T cells leave the thymus in an anergizable state.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000581-01
Application #
3809743
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Kamala, Tirumalai; Nanda, Navreet K (2009) Protective response to Leishmania major in BALB/c mice requires antigen processing in the absence of DM. J Immunol 182:4882-90
Reines, Brandon; Cheng, Lily I; Matzinger, Polly (2009) Unexpected regeneration in middle-aged mice. Rejuvenation Res 12:45-52
Guzman, Valeska B; Yambartsev, Anatoly; Goncalves-Primo, Amador et al. (2008) New approach reveals CD28 and IFNG gene interaction in the susceptibility to cervical cancer. Hum Mol Genet 17:1838-44
Helft, Julie; Jacquet, Alexandra; Joncker, Nathalie T et al. (2008) Antigen-specific T-T interactions regulate CD4 T-cell expansion. Blood 112:1249-58
Kamala, T (2008) An optimized immunomagnetic bead-based negative selection protocol for CD4 T-cell isolation from mouse lymph nodes and spleen. Scand J Immunol 67:285-94
Usharauli, David; Kamala, Tirumalai (2008) Brief antigenic stimulation generates effector CD8 T cells with low cytotoxic activity and high IL-2 production. J Immunol 180:4507-13
Chan, William F N; Perez-Diez, Ainhoa; Razavy, Haide et al. (2007) The ability of natural tolerance to be applied to allogeneic tissue: determinants and limits. Biol Direct 2:10
Perez-Diez, Ainhoa; Joncker, Nathalie T; Choi, Kyungho et al. (2007) CD4 cells can be more efficient at tumor rejection than CD8 cells. Blood 109:5346-54
Perez-Diez, Ainhoa; Morgun, Andrey; Shulzhenko, Natalia (2007) Microarrays for cancer diagnosis and classification. Adv Exp Med Biol 593:74-85
Matzinger, Polly (2007) Friendly and dangerous signals: is the tissue in control? Nat Immunol 8:11-3

Showing the most recent 10 out of 31 publications