Abstract

We have devised a new method to measure cytotoxic T cell activities in vitro and perfected a method to measure immune rejection in vivo. using these techniques in combination with our standard thymic organ culture, we are in the process of studying four questions. 1. Do newly born T cells have the thymus in a tolerizable or activateable state. 2. Does thymic epithelium present antigens in a tolerogenic or immunogenic fashion. 3. Does T cell priming and/or memory require the presence of B cells. 4. What is the frequency of alloreactive T cells. Our preliminary experiments suggest that newly developed CD4+ and CD8+ T cells are fully capable of responding to allogeneic stimulators, thus refuting models that require the assumption that T cells leave the thymus is an anergizable state.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000581-02
Application #
3803262
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Kamala, Tirumalai; Nanda, Navreet K (2009) Protective response to Leishmania major in BALB/c mice requires antigen processing in the absence of DM. J Immunol 182:4882-90
Reines, Brandon; Cheng, Lily I; Matzinger, Polly (2009) Unexpected regeneration in middle-aged mice. Rejuvenation Res 12:45-52
Guzman, Valeska B; Yambartsev, Anatoly; Goncalves-Primo, Amador et al. (2008) New approach reveals CD28 and IFNG gene interaction in the susceptibility to cervical cancer. Hum Mol Genet 17:1838-44
Helft, Julie; Jacquet, Alexandra; Joncker, Nathalie T et al. (2008) Antigen-specific T-T interactions regulate CD4 T-cell expansion. Blood 112:1249-58
Kamala, T (2008) An optimized immunomagnetic bead-based negative selection protocol for CD4 T-cell isolation from mouse lymph nodes and spleen. Scand J Immunol 67:285-94
Usharauli, David; Kamala, Tirumalai (2008) Brief antigenic stimulation generates effector CD8 T cells with low cytotoxic activity and high IL-2 production. J Immunol 180:4507-13
Perez-Diez, Ainhoa; Joncker, Nathalie T; Choi, Kyungho et al. (2007) CD4 cells can be more efficient at tumor rejection than CD8 cells. Blood 109:5346-54
Perez-Diez, Ainhoa; Morgun, Andrey; Shulzhenko, Natalia (2007) Microarrays for cancer diagnosis and classification. Adv Exp Med Biol 593:74-85
Matzinger, Polly (2007) Friendly and dangerous signals: is the tissue in control? Nat Immunol 8:11-3
Kamala, T (2007) Hock immunization: a humane alternative to mouse footpad injections. J Immunol Methods 328:204-14

Showing the most recent 10 out of 31 publications