The focus of this work has been to understand the molecular details that control the interaction of MHC molecules with NK receptors, T cell receptors, and with coreceptors such as CD8. These studies are dependent upon structural, functional, and biophysical analysis of the interaction of the molecules in question, and attempts are made to correlate binding properties with function and structure. Progress in the past year has been particularly fruitful with respect to: 1) the binding analysis of a large panel of MHC-I, beta2-microglobulin, and NK receptor (Ly49A) site directed mutants that identify the major site of interaction to a large region that includes residues both of the MHC and of the beta2-m light chain; and 2) binding studies of Ly49C with H-2Kb. These studies have resolved a major controversy concerning the site of interaction of the murine lectin-like NK receptors with MHC-I and raise additional questions as to how receptors with limited, but degenerate specificity function. In addition, the determination of the X-ray structure of the NK receptor, Ly49I, which has distinct specificity from Ly49C and Ly49A offers a glimpse of the plasticity of these molecules. Crystals obtained from complexes of H-2Kb with Ly49C suggest that a structure determination of this distinct MHC/receptor complex may be in sight.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000622-11
Application #
6669526
Study Section
(LI)
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code
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