Asthma is an inflammatory disease of the airways. After allergen exposure, IgE bearing cells, such as mast cells and basophils, are first activated. Later, T lymphocytes enter the airways, are activated by allergen and play a key role in regulating this inflammatory response through the elaboration of cytokines such as IL-4, IL-5 and IFN-gamma. This project seeks to examine the cytokine and mediator profiles of mast cell, basophil and T cell subpopulations that may be involved in the generation of inflammation in asthmatic airways. We have developed sensitive intracellular cytokine assays to measure allergen specific T cell cytokine responses almost directly ex vivo. Using these methods, we have shown that allergen specific T cells are predominantly of the classic Th2 phenotype and that on the single cell level IL-4, IL-5 and IL-13 are highly co-expressed. Interestingly, IL-2, which is often considered a Th1 cytokine was also highly coexpressed. Allergen specific T cell expressed high levels of the CCR4 and CRTH2 chemoattractant receptors. These results provide a detailed view of the T cell response to allergens with a fidelity heretofore not possible.
| Liu, Rebecca Berlant; Engels, Boris; Schreiber, Karin et al. (2013) IL-15 in tumor microenvironment causes rejection of large established tumors by T cells in a noncognate T cell receptor-dependent manner. Proc Natl Acad Sci U S A 110:8158-63 |
| Liu, Rebecca B; Engels, Boris; Arina, Ainhoa et al. (2012) Densely granulated murine NK cells eradicate large solid tumors. Cancer Res 72:1964-74 |
| Prussin, Calman; Metcalfe, Dean D (2006) 5. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 117:S450-6 |
| Fritsch, Ruth D; Shen, Xinglei; Illei, Gabor G et al. (2006) Abnormal differentiation of memory T cells in systemic lupus erythematosus. Arthritis Rheum 54:2184-97 |
| Garvey, Tara L; Dyer, Kimberly D; Ellis, John A et al. (2005) Inflammatory responses to pneumovirus infection in IFN-alpha beta R gene-deleted mice. J Immunol 175:4735-44 |
| Foroughi, Shabnam; Prussin, Calman (2005) Clinical management of eosinophilic gastrointestinal disorders. Curr Allergy Asthma Rep 5:259-61 |
| Prussin, Calman; Metcalfe, Dean D (2003) 4. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 111:S486-94 |
| Prussin, Calman; Griffith, Daniel T; Boesel, Kevin M et al. (2003) Omalizumab treatment downregulates dendritic cell FcepsilonRI expression. J Allergy Clin Immunol 112:1147-54 |
| Foster, Barbara; Metcalfe, Dean D; Prussin, Calman (2003) Human dendritic cell 1 and dendritic cell 2 subsets express FcepsilonRI: correlation with serum IgE and allergic asthma. J Allergy Clin Immunol 112:1132-8 |
| Metcalfe, Dean D (2003) Introduction: what are the issues in addressing the allergenic potential of genetically modified foods? Environ Health Perspect 111:1110-3 |
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