Abstract

The previously developed pulsatility analysis algorithm (PULSEFIT) was applied in several additional studies. In one small frequent peaks of luteinizing hormone (LH) could be discerned reliably and confirmed with an assay for the alpha subunit of LH. Pulsatility analysis was also conducted in an ongoing study of normal children in collaboration with researchers at EPA. A mathematical model explaining apparent cooperative binding of estrogen to receptor was extended to account for the temperature dependence of the cooperative phenomena. The model postulates formation of a heterodimer of receptor. A novel explanation of drug efficacy and negative antagonism in terms of a ternary-complex model was produced. The model explains the sodium effect and certain other observations for G-protein mediated cell surface receptors such as the opiate receptor. Statistical consultations with many investigators at NIH were undertaken in support of statistical problems in ligand-binding, receptor modelling, kinetic modeling, immunoassay, and dose-response analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Center for Information Technology (CIT)
Type
Intramural Research (Z01)
Project #
1Z01CT000227-02
Application #
3838538
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Center for Information Technology
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Pajevic, Sinisa; Plenz, Dietmar (2009) Efficient network reconstruction from dynamical cascades identifies small-world topology of neuronal avalanches. PLoS Comput Biol 5:e1000271
McQueen, Philip G; McKenzie, F Ellis (2008) Host control of malaria infections: constraints on immune and erythropoeitic response kinetics. PLoS Comput Biol 4:e1000149
Coppey, Mathieu; Boettiger, Alistair N; Berezhkovskii, Alexander M et al. (2008) Nuclear trapping shapes the terminal gradient in the Drosophila embryo. Curr Biol 18:915-9
Sam, Vichetra; Tai, Chin-Hsien; Garnier, Jean et al. (2008) Towards an automatic classification of protein structural domains based on structural similarity. BMC Bioinformatics 9:74
Hendler, Richard W; Shrager, Richard I; Meuse, Curtis W (2008) The ability of actinic light to modify the bacteriorhodopsin photocycle revisited: heterogeneity vs photocooperativity. Biochemistry 47:5406-16
Williams, Ruth R E; Azuara, Veronique; Perry, Pascale et al. (2006) Neural induction promotes large-scale chromatin reorganisation of the Mash1 locus. J Cell Sci 119:132-40
Nishizuka, Satoshi; Washburn, Newell R; Munson, Peter J (2006) Evaluation method of ordinary flatbed scanners for quantitative density analysis. Biotechniques 40:442, 444, 446 passim
McQueen, Philip G; McKenzie, F Ellis (2006) Competition for red blood cells can enhance Plasmodium vivax parasitemia in mixed-species malaria infections. Am J Trop Med Hyg 75:112-25
Sam, Vichetra; Tai, Chin-Hsien; Garnier, Jean et al. (2006) ROC and confusion analysis of structure comparison methods identify the main causes of divergence from manual protein classification. BMC Bioinformatics 7:206
Knodler, Leigh A; Steele-Mortimer, Olivia (2005) The Salmonella effector PipB2 affects late endosome/lysosome distribution to mediate Sif extension. Mol Biol Cell 16:4108-23

Showing the most recent 10 out of 15 publications