Abstract

Bone cells derived from a variety of animal species including human, bovine, ovine and rodent, and of varying developmental ages, have been utilized to: 1) study the biosynthesis and deposition of extracellular matrix proteins such as collagen, osteonectin, bone proteoglycans and other bone proteins, and alterations of matrix production in the disease Osteogenesis Imperfecta; 2) study the responsiveness of the cells to a variety of hormonal and pharacoloical factors (such as estrogen and fluoride); 3) elucidate the production and interaction of growth factors (such as TGF-beta, insulin-like growth factors and platelet-derived growth factors); 4) study the potential function of bone matrix proteins through the use of functional assays and the use of anti-sense DNA to inhibit specific protein synthesis; and 5) serve as a source of mRNA and DNA for studies of the proteins at the genomic level.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000380-06
Application #
3875212
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Institute of Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Padmanabhan, Raji; Chen, Kevin G; Gillet, Jean-Pierre et al. (2012) Regulation and expression of the ATP-binding cassette transporter ABCG2 in human embryonic stem cells. Stem Cells 30:2175-87
Balakumaran, Arun; Robey, Pamela Gehron; Fedarko, Neal et al. (2010) Bone marrow microenvironment in myelomagenesis: its potential role in early diagnosis. Expert Rev Mol Diagn 10:465-80
Mazar, Julia; Thomas, Molly; Bezrukov, Ludmila et al. (2009) Cytotoxicity mediated by the Fas ligand (FasL)-activated apoptotic pathway in stem cells. J Biol Chem 284:22022-8
Pawelczyk, Edyta; Jordan, Elaine K; Balakumaran, Arun et al. (2009) In vivo transfer of intracellular labels from locally implanted bone marrow stromal cells to resident tissue macrophages. PLoS One 4:e6712
Inkson, Colette A; Ono, Mitsuaki; Kuznetsov, Sergei A et al. (2008) TGF-beta1 and WISP-1/CCN-4 can regulate each other's activity to cooperatively control osteoblast function. J Cell Biochem 104:1865-78
Tsutsui, T W; Riminucci, M; Holmbeck, Kenn et al. (2008) Development of craniofacial structures in transgenic mice with constitutively active PTH/PTHrP receptor. Bone 42:321-31
Pawelczyk, Edyta; Arbab, Ali S; Chaudhry, Aneeka et al. (2008) In vitro model of bromodeoxyuridine or iron oxide nanoparticle uptake by activated macrophages from labeled stem cells: implications for cellular therapy. Stem Cells 26:1366-75
Bianco, Paolo; Robey, Pamela Gehron; Simmons, Paul J (2008) Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2:313-9
Robey, Pamela Gehron; Kuznetsov, Sergei A; Riminucci, Mara et al. (2007) Skeletal (""mesenchymal"") stem cells for tissue engineering. Methods Mol Med 140:83-99
Feuillan, Penelope; Calis, Karim; Hill, Suvimol et al. (2007) Letrozole treatment of precocious puberty in girls with the McCune-Albright syndrome: a pilot study. J Clin Endocrinol Metab 92:2100-6

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