Abstract

Cartilage is a highly specialized tissue which functions to resist compression and to absorb shock. The purpose of this project is to understand molecular mechanisms by which genes for cartilage components are regulated and expressed during normal development and in disease states. The alteration of cartilage matrix protein are likely to be associated with human diseases such as osteoporosis, osteoarthritis, and rheumatoid arthritis. We have determined the primary structure of some of the cartilage components. We have also isolated and characterized genes for these proteins. DNA elements which regulate these genes have been identified and nuclear protein factors bound to them have been characterized. Structure and function relationship has been studied using expression vectors and synthetic peptide approaches. DNA prepared from patients with chondrodysplasia has been screened to examine their linkage to cartilage genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000483-02
Application #
3875256
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Institute of Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Ishikawa, Masaki; Williams, Geneva L; Ikeuchi, Tomoko et al. (2016) Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns. J Cell Sci 129:1018-30
Nakamura, Takashi; Yoshitomi, Yasuo; Sakai, Kiyoshi et al. (2014) Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation. J Cell Sci 127:5261-72
Talamillo, Ana; Delgado, Irene; Nakamura, Takashi et al. (2010) Role of Epiprofin, a zinc-finger transcription factor, in limb development. Dev Biol 337:363-74
Matsunobu, Tomoya; Torigoe, Kiyoyuki; Ishikawa, Masaki et al. (2009) Critical roles of the TGF-beta type I receptor ALK5 in perichondrial formation and function, cartilage integrity, and osteoblast differentiation during growth plate development. Dev Biol 332:325-38
Tsang, Kwok Yeung; Chan, Danny; Cheslett, Deborah et al. (2007) Surviving endoplasmic reticulum stress is coupled to altered chondrocyte differentiation and function. PLoS Biol 5:e44
de Vega, Susana; Iwamoto, Tsutomu; Nakamura, Takashi et al. (2007) TM14 is a new member of the fibulin family (fibulin-7) that interacts with extracellular matrix molecules and is active for cell binding. J Biol Chem 282:30878-88
Iwamoto, Masahiro; Tamamura, Yoshihiro; Koyama, Eiki et al. (2007) Transcription factor ERG and joint and articular cartilage formation during mouse limb and spine skeletogenesis. Dev Biol 305:40-51
Matsumoto, Kazu; Kamiya, Nobuhiro; Suwan, Keittisak et al. (2006) Identification and characterization of versican/PG-M aggregates in cartilage. J Biol Chem 281:18257-63
Hozumi, Kentaro; Suzuki, Nobuharu; Nielsen, Peter K et al. (2006) Laminin alpha1 chain LG4 module promotes cell attachment through syndecans and cell spreading through integrin alpha2beta1. J Biol Chem 281:32929-40
Tamamura, Yoshihiro; Otani, Tomohiro; Kanatani, Naoko et al. (2005) Developmental regulation of Wnt/beta-catenin signals is required for growth plate assembly, cartilage integrity, and endochondral ossification. J Biol Chem 280:19185-95

Showing the most recent 10 out of 16 publications