Noncovalent Intermolecular Interactions in Biochemistry

Minton, A P.

Abstract

The mechanism by means of which volume-sensitive membrane ion transporters respond to alterations in intracellular content has been studied experimentally and theoretically. A computer program for rapid simulation of many types of experiments that may be performed on the analytical ultracentrifuge has been developed. A new technique for quantification of concentration gradients of multiple proteins in a mixture at sedimentation equilibrium has been developed. Computer programs employing novel algorithms for the global analysis of multiple absorbance gradients of two component solutions at sedimentation equilibrium in the context of models for self- and heteroassociation have been developed.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK024150-23
• Application #: 3754081
• Support Year: 23
• Fiscal Year: 1994

Institution

• Name: National Institute of Diabetes and Digestive and Kidney Diseases
• Country: United States

Publications

Minton, Allen P (2006) Macromolecular crowding. Curr Biol 16:R269-71

Howlett, Geoffrey J; Minton, Allen P; Rivas, German (2006) Analytical ultracentrifugation for the study of protein association and assembly. Curr Opin Chem Biol 10:430-6

Minton, Allen P (2006) How can biochemical reactions within cells differ from those in test tubes? J Cell Sci 119:2863-9

Kameyama, Keiichi; Minton, Allen P (2006) Rapid quantitative characterization of protein interactions by composition gradient static light scattering. Biophys J 90:2164-9

Ellis, R John; Minton, Allen P (2006) Protein aggregation in crowded environments. Biol Chem 387:485-97

McPhie, Peter; Ni, Yi-sheng; Minton, Allen P (2006) Macromolecular crowding stabilizes the molten globule form of apomyoglobin with respect to both cold and heat unfolding. J Mol Biol 361:7-10

Gonzalez, Jose Manuel; Velez, Marisela; Jimenez, Mercedes et al. (2005) Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils. Proc Natl Acad Sci U S A 102:1895-900

Minton, Allen P (2005) Models for excluded volume interaction between an unfolded protein and rigid macromolecular cosolutes: macromolecular crowding and protein stability revisited. Biophys J 88:971-85

Attri, Arun K; Minton, Allen P (2005) Composition gradient static light scattering: a new technique for rapid detection and quantitative characterization of reversible macromolecular hetero-associations in solution. Anal Biochem 346:132-8

Attri, Arun K; Minton, Allen P (2005) New methods for measuring macromolecular interactions in solution via static light scattering: basic methodology and application to nonassociating and self-associating proteins. Anal Biochem 337:103-10