Enzyme Structure

Davies, D R.

Abstract

The multi-enzyme complex, tryptophan synthase from Salmonella typhimurium, has been further analyzed by X-ray diffraction. High resolution data have been collected for two derivatives of a mutant, K87T, of the beta subunit that approximate enzyme catalyzed intermediates. These structures have been refined, to provide new information about the disposition of residues and their interaction with the intermediates of the beta reaction. In addition another mutant, alpha60E, has been analyzed and data have been collected for two inhibitor complexes of the betaK87T mutant to examine the effect on flexible regions of the alpha active site.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK034002-29
• Application #: 3776343
• Support Year: 29
• Fiscal Year: 1993

Institution

• Name: National Institute of Diabetes and Digestive and Kidney Diseases
• Country: United States

Publications

Liu, Lin; Botos, Istvan; Wang, Yan et al. (2008) Structural basis of toll-like receptor 3 signaling with double-stranded RNA. Science 320:379-81

Leonard, Joshua N; Ghirlando, Rodolfo; Askins, Janine et al. (2008) The TLR3 signaling complex forms by cooperative receptor dimerization. Proc Natl Acad Sci U S A 105:258-63

Bell, Jessica K; Askins, Janine; Hall, Pamela R et al. (2006) The dsRNA binding site of human Toll-like receptor 3. Proc Natl Acad Sci U S A 103:8792-7

Cohen, Gerson H; Silverton, Enid W; Padlan, Eduardo A et al. (2005) Water molecules in the antibody-antigen interface of the structure of the Fab HyHEL-5-lysozyme complex at 1.7 A resolution: comparison with results from isothermal titration calorimetry. Acta Crystallogr D Biol Crystallogr 61:628-33

Bell, Jessica K; Botos, Istvan; Hall, Pamela R et al. (2005) The molecular structure of the Toll-like receptor 3 ligand-binding domain. Proc Natl Acad Sci U S A 102:10976-80

Bell, Jessica K; Mullen, Gregory E D; Leifer, Cynthia A et al. (2003) Leucine-rich repeats and pathogen recognition in Toll-like receptors. Trends Immunol 24:528-33

Jhee, K H; Yoshimura, T; Miles, E W et al. (2000) Stereochemistry of the transamination reaction catalyzed by aminodeoxychorismate lyase from Escherichia coli: close relationship between fold type and stereochemistry. J Biochem (Tokyo) 128:679-86

Jhee, K H; McPhie, P; Miles, E W (2000) Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39:10548-56

Hickman, A B; Li, Y; Mathew, S V et al. (2000) Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Mol Cell 5:1025-34

Fan, Y X; McPhie, P; Miles, E W (2000) Regulation of tryptophan synthase by temperature, monovalent cations, and an allosteric ligand. Evidence from Arrhenius plots, absorption spectra, and primary kinetic isotope effects. Biochemistry 39:4692-703