Non-Heritable Antibiotic Resistance

Rosner, J L.

Abstract

Our previous work showed that certain weak acids, especially aspirin and salicylate, induce nonheritable resistance to ampicillin, chloramphenicol, nalidixic acid and tetracycline in Escherichia coli. Similar results have now been obtained in various other bacteria including certain Bacillus, Klebsiella, Salmonella, Shigella and Vibrio, but not Streptococcus or Staphylococcus species. Furthermore, acetaminophen (Tylenol) has also been found to induce noninheritable drug resistance E. coli. Several additional effects of salicylate on E. coli have been demonstrated. In the presence of salicylate, the bacteria require methionine or isoleucine and valine, appear to be Lac-, and are hypersensitive to kanamycin. Mutants that do not show these traits are being isolated and characterized.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK036003-02
• Application #: 3964702
• Support Year: 2
• Fiscal Year: 1986

Institution

• Name: U.S. National Inst Diabetes/Digst/Kidney
• Country: United States

Publications

Rosner, Judah L; Martin, Robert G (2009) An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants. J Bacteriol 191:5283-92

Zhang, Aixia; Rosner, Judah L; Martin, Robert G (2008) Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli. Mol Microbiol 69:1450-5

Martin, Robert G; Bartlett, Emily S; Rosner, Judah L et al. (2008) Activation of the Escherichia coli marA/soxS/rob regulon in response to transcriptional activator concentration. J Mol Biol 380:278-84

Kawano, Mitsuoki; Storz, Gisela; Rao, B Sridhar et al. (2005) Detection of low-level promoter activity within open reading frame sequences of Escherichia coli. Nucleic Acids Res 33:6268-76

Martin, Robert G; Rosner, Judah L (2004) Transcriptional and translational regulation of the marRAB multiple antibiotic resistance operon in Escherichia coli. Mol Microbiol 53:183-91

Dangi, Bindi; Gronenborn, Angela M; Rosner, Judah L et al. (2004) Versatility of the carboxy-terminal domain of the alpha subunit of RNA polymerase in transcriptional activation: use of the DNA contact site as a protein contact site for MarA. Mol Microbiol 54:45-59

Thomason, Lynn C; Court, Donald L; Datta, Atin R et al. (2004) Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase. J Bacteriol 186:8248-53

Martin, Robert G; Rosner, Judah L (2003) Analysis of microarray data for the marA, soxS, and rob regulons of Escherichia coli. Methods Enzymol 370:278-80

Rosner, Judah L; Dangi, Bindi; Gronenborn, Angela M et al. (2002) Posttranscriptional activation of the transcriptional activator Rob by dipyridyl in Escherichia coli. J Bacteriol 184:1407-16

Martin, Robert G; Gillette, William K; Martin, Nicholas I et al. (2002) Complex formation between activator and RNA polymerase as the basis for transcriptional activation by MarA and SoxS in Escherichia coli. Mol Microbiol 43:355-70