We have continued our studies of the insulin-like growth factors (IGFs), their receptors and carrier proteins. During the past year we have demonstrated that: (1) multiple IGF-II RNAs (1.2 to 4.6 kb) arise from a single gene through the use of 2 promoters and 2 polyA addition sites; (2) the IGF-II gene is transcribed from both promoters in 11 fetal rat tissues; (3) IGF-II RNA in different tissues is translated into pre-pro-rIGF-II is processed to 7.5 kDa IGF-II; (4) the fetal/neonatal form of the IGF carrier protein has been cloned; (5) the carrier protein precursor contains a 34 amino acid prepeptide and the 270 amino acid mature protein; (6) carrier protein mRNA is expressed in multiple fetal tissues, but is decreased in the corresponding adult tissues; (7) polyclonal antibodies to the purified type II IGF receptor do not stimulate or inhibit IGF actions in L6 rat myoblasts, suggesting that these effects are mediated by the type I rather than the type II receptor, (8) circulating type II IGF receptors corresponding to the extracellular domain are present in fetal and neonatal rat serum; (9) levels of the circulating type II receptor decrease markedly in older rats; (10) activation of human T lymphocytes results in increased expression of type I and type II IGF receptors, suggesting that the IGFs may participate in the activation cascade; (11) two-chain insulin-IGF hybrid molecules containing the A-domain of IGF-I have increased mitogenic activity and binding to type I IGF receptors but do not bind to IGF carrier proteins.
Adams, Ted D; Davidson, Lance E; Litwin, Sheldon E et al. (2017) Weight and Metabolic Outcomes 12 Years after Gastric Bypass. N Engl J Med 377:1143-1155 |
Adams, Ted D; Davidson, Lance E; Litwin, Sheldon E et al. (2012) Health benefits of gastric bypass surgery after 6 years. JAMA 308:1122-31 |
Kolotkin, Ronette L; Davidson, Lance E; Crosby, Ross D et al. (2012) Six-year changes in health-related quality of life in gastric bypass patients versus obese comparison groups. Surg Obes Relat Dis 8:625-33 |
Hammoud, Ahmad O; Walker, James M; Gibson, Mark et al. (2011) Sleep apnea, reproductive hormones and quality of sexual life in severely obese men. Obesity (Silver Spring) 19:1118-23 |
Kolotkin, Ronette L; LaMonte, Michael J; Litwin, Sheldon et al. (2011) Cardiorespiratory fitness and health-related quality of life in bariatric surgery patients. Obes Surg 21:457-64 |
Wasmund, Stephen L; Owan, Theophilus; Yanowitz, Frank G et al. (2011) Improved heart rate recovery after marked weight loss induced by gastric bypass surgery: two-year follow up in the Utah Obesity Study. Heart Rhythm 8:84-90 |
Hunt, Steven C; Hasstedt, Sandra J; Xin, Yuanpei et al. (2011) Polymorphisms in the NPY2R gene show significant associations with BMI that are additive to FTO, MC4R, and NPFFR2 gene effects. Obesity (Silver Spring) 19:2241-7 |
Benraouane, Fethi; Litwin, Sheldon E (2011) Reductions in cardiovascular risk after bariatric surgery. Curr Opin Cardiol 26:555-61 |
Kollerits, Barbara; Coassin, Stefan; Kiechl, Stefan et al. (2010) A common variant in the adiponutrin gene influences liver enzyme values. J Med Genet 47:116-9 |
Adams, Ted D; Pendleton, Robert C; Strong, Michael B et al. (2010) Health outcomes of gastric bypass patients compared to nonsurgical, nonintervened severely obese. Obesity (Silver Spring) 18:121-30 |
Showing the most recent 10 out of 22 publications