Abstract

It has been postulated that in vivo toxicity of asbestos and air pollution particles results from the catalysis of free radical generation. Exposure to these lung toxins can be associated with increased human morbidity and mortality. However, molecular mehcanisms of lung injury remain unknown. We examined in vivo free radical generation after exposure to asbestos or air pollution particles by using electron spin resonance (ESR) spin trapping technique. ESR spectroscopy of the chloroform extract from lungs exposed to asbestos or air pollution particles gave a spectrum consistent with a carbon-centered radical adduct, while those spectra from control lungs revealed a much weaker signal. The radical adducts detected by ESR are very similar to, if not identical, with ethyl and penthyl radical adducts, providing for the first time ESR evidence of in vivo lipid peroxidation resulting from asbestos and air pollution particle exposure. In addition, the generation of free radicals in lung tissue appears to be associated with soluble metals present in the air pollution particles. Understanding the source of radical species could lead to an understanding of the mechanisms underlying clinical pathology in patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES050117-10
Application #
6432347
Study Section
(LPC)
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Liu, Jie; Qian, Steven Y; Guo, Qiong et al. (2008) Cadmium generates reactive oxygen- and carbon-centered radical species in rats: insights from in vivo spin-trapping studies. Free Radic Biol Med 45:475-81
Woods, Courtney G; Burns, Amanda M; Maki, Akira et al. (2007) Sustained formation of alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone radical adducts in mouse liver by peroxisome proliferators is dependent upon peroxisome proliferator-activated receptor-alpha, but not NADPH oxidase. Free Radic Biol Med 42:335-42
Jiang, JinJie; Corbett, Jean; Hogg, Neil et al. (2007) An electron paramagnetic resonance investigation of the oxygen dependence of the arterial-venous gradient of nitrosyl hemoglobin in blood circulation. Free Radic Biol Med 43:1208-15
Nakai, Kozo; Kadiiska, Maria B; Jiang, Jin-Jie et al. (2006) Free radical production requires both inducible nitric oxide synthase and xanthine oxidase in LPS-treated skin. Proc Natl Acad Sci U S A 103:4616-21
Kono, Hiroshi; Woods, Courtney G; Maki, Akira et al. (2006) Electron spin resonance and spin trapping technique provide direct evidence that edaravone prevents acute ischemia-reperfusion injury of the liver by limiting free radical-mediated tissue damage. Free Radic Res 40:579-88
Zhong, Zhi; Connor, Henry D; Li, Xiangli et al. (2006) Reduction of ciclosporin and tacrolimus nephrotoxicity by plant polyphenols. J Pharm Pharmacol 58:1533-43
Arimoto, Toyoko; Kadiiska, Maria B; Sato, Keizo et al. (2005) Synergistic production of lung free radicals by diesel exhaust particles and endotoxin. Am J Respir Crit Care Med 171:379-87
Zhong, Zhi; Connor, Henry D; Froh, Mattias et al. (2005) Free radical-dependent dysfunction of small-for-size rat liver grafts: prevention by plant polyphenols. Gastroenterology 129:652-64
Yue Qian, Steven; Kadiiska, Maria B; Guo, Qiong et al. (2005) A novel protocol to identify and quantify all spin trapped free radicals from in vitro/in vivo interaction of HO(.-) and DMSO: LC/ESR, LC/MS, and dual spin trapping combinations. Free Radic Biol Med 38:125-35
Kadiiska, M B; Ghio, A J; Mason, R P (2004) ESR investigation of the oxidative damage in lungs caused by asbestos and air pollution particles. Spectrochim Acta A Mol Biomol Spectrosc 60:1371-7

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