Abstract

In the toad retinal pigment epithelium (RPE) eyecup, it was demonstrated that the interphotoreceptor retinoid-binding protein (IRBP) promotes the RPE's conversion of radiolabeled 11-cis retinyl ester to 11-cis retinal. IRBP was successfully photoaffinity-labeled with tritiated retinoic acid, and the photoaffinity labeled IRBP was used to demonstrate the presence of a putative cell surface receptor for IRBP in RPE cells in culture. Binding affinities of rat B cell and human B cell heat shock protein (hsp) 70s to analogs of rat IRBP peptide 1181-1191 were demonstrated to be correlated with the pathogenicity of the peptides in the Lewis rat. An examination of the mechanism of photoreceptor cell death in the vitiligo mouse model of retinal degeneration showed that an apoptotic mechanism is involved. It was also shown that the expression of TRPM-2 (Clusterin) is elevated in vitiligo retinas, beginning at one week in the RPE and later (by 12 weeks) in the neural retina. Drosophila retinoid- and fatty acid-binding protein (DRBP) wa localized to the cone (Semper) cells in the Drosophila compound eye. DRBP levels were lower in the eyes of flies that were deprived of carotenoids, suggesting that DRBP is relevant to retinoid handling in the Drosophila eye.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000070-18
Application #
3734009
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Cortes, Lizette M; Mattapallil, Mary J; Silver, Phyllis B et al. (2008) Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice. Invest Ophthalmol Vis Sci 49:1946-56
Wu, Qingqing; Blakeley, Lorie R; Cornwall, M Carter et al. (2007) Interphotoreceptor retinoid-binding protein is the physiologically relevant carrier that removes retinol from rod photoreceptor outer segments. Biochemistry 46:8669-79
Duncan, Todd; Wiggert, Barbara; Whittaker, Noel et al. (2006) Effect of visible light on normal and P23H-3 transgenic rat retinas: characterization of a novel retinoic acid derivative present in the P23H-3 retina. Photochem Photobiol 82:741-5
Ala-Laurila, Petri; Kolesnikov, Alexander V; Crouch, Rosalie K et al. (2006) Visual cycle: Dependence of retinol production and removal on photoproduct decay and cell morphology. J Gen Physiol 128:153-69
Kutty, R Krishnan; Chen, Shanyi; Samuel, William et al. (2006) Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein. Biochem Biophys Res Commun 345:1333-41
Kutty, R Krishnan; Samuel, William; Chen, Shanyi et al. (2006) Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Muller and ganglion cells. Neurosci Lett 404:294-8
Qtaishat, Nasser M; Wiggert, Barbara; Pepperberg, David R (2005) Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination. Exp Eye Res 81:455-63
Tsina, Efthymia; Chen, Chunhe; Koutalos, Yiannis et al. (2004) Physiological and microfluorometric studies of reduction and clearance of retinal in bleached rod photoreceptors. J Gen Physiol 124:429-43
Cornwall, M Carter; Tsina, Efthymia; Crouch, Rosalie K et al. (2003) Regulation of the visual cycle: retinol dehydrogenase and retinol fluorescence measurements in vertebrate retina. Adv Exp Med Biol 533:353-60
Wang, Peng; Sun, Shu-Hui; Silver, Phyllis B et al. (2003) Methimazole protects from experimental autoimmune uveitis (EAU) by inhibiting antigen presenting cell function and reducing antigen priming. J Leukoc Biol 73:57-64

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