Abstract

The NEI Central Transgenic Animal Facility is a research support facility for all NEI intramural researchers requiring the use of transgenic mice in their research programs. We are currently providing transgenic animal support to researchers from five laboratories in the NEI (LI, LMDB, LMOD, LRCMB, OGSCB); in our program, there are currently 153 DNA constructs at various stages of completion. NEI researchers using molecular biology techniques to study the eye submit DNA constructs to our section for production of transgenic mice. We create transgenic mice by standard procedures, then biopsy and perform DNA analysis on the mice that are born from these procedures to identify transgene-positive mice. At researchers' request, we mate positive transgenic mice, wean litters, biopsy and analyze DNA from successive generations of transgenic mice, provide the transgenic animals to researchers for use in their experiments, and cryo-preserve and bank embryos from important mouse lines (both transgenic and naturally occurring) for long-term storage.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000285-04
Application #
2574520
Study Section
Special Emphasis Panel (LMDB)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Cortes, Lizette M; Mattapallil, Mary J; Silver, Phyllis B et al. (2008) Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice. Invest Ophthalmol Vis Sci 49:1946-56
Kreslova, Jana; Machon, Ondrej; Ruzickova, Jana et al. (2007) Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function. Genesis 45:157-68
Fujimoto, Chiaki; Yu, Cheng-Rong; Shi, Guangpu et al. (2006) Pertussis toxin is superior to TLR ligands in enhancing pathogenic autoimmunity, targeted at a neo-self antigen, by triggering robust expansion of Th1 cells and their cytokine production. J Immunol 177:6896-903
Chen, Jun; Fujimoto, Chiaki; Vistica, Barbara P et al. (2006) Active participation of antigen-nonspecific lymphoid cells in immune-mediated inflammation. J Immunol 177:3362-8
Takase, Hiroshi; Yu, Cheng-Rong; Ham, Don-Il et al. (2006) Inflammatory processes triggered by TCR engagement or by local cytokine expression: differences in profiles of gene expression and infiltrating cell populations. J Leukoc Biol 80:538-45
Senatorov, Vladimir; Malyukova, Irina; Fariss, Robert et al. (2006) Expression of mutated mouse myocilin induces open-angle glaucoma in transgenic mice. J Neurosci 26:11903-14
Zeng, Yong; Takada, Yuichiro; Kjellstrom, Sten et al. (2004) RS-1 Gene Delivery to an Adult Rs1h Knockout Mouse Model Restores ERG b-Wave with Reversal of the Electronegative Waveform of X-Linked Retinoschisis. Invest Ophthalmol Vis Sci 45:3279-85
Maddala, Rupalatha; Deng, Pei-Feng; Costello, Joseph M et al. (2004) Impaired cytoskeletal organization and membrane integrity in lens fibers of a Rho GTPase functional knockout transgenic mouse. Lab Invest 84:679-92
Ham, Don-Il; Kim, Stephen J; Chen, Jun et al. (2004) Central immunotolerance in transgenic mice expressing a foreign antigen under control of the rhodopsin promoter. Invest Ophthalmol Vis Sci 45:857-62
Vinores, S A; Xiao, W-H; Zimmerman, R et al. (2003) Upregulation of vascular endothelial growth factor (VEGF) in the retinas of transgenic mice overexpressing interleukin-1beta (IL-1beta) in the lens and mice undergoing retinal degeneration. Histol Histopathol 18:797-810

Showing the most recent 10 out of 22 publications