Molecular Genetics of Early Eye Development

Tomarev, Stanislav I.

Abstract

The role of homeobox gene, Prox 1 in vertebrate eye development and regeneration was studied. The zebrafish Prox 1 gene was isolated and characterized and its expression pattern was studied by whole-mount immunohistochemistry and in situ hybridization. Expression of Prox 1 was first detected in the ten-somite stage embryos in the ectoderm overlaying the eye buds. As embryogenesis proceeds, Prox 1 expression was also detected in muscle pioneer cells, forebrain-midbrain boundary, ventral hindbrain, and selected cells of the ventral spinal cord. A partial Prox 1 cDNA was isolated from the newt. In the process of lens regeneration in the newt, Prox 1 protein was found to be selectively expressed in the regenerating competent cells of the dorsal iris but not in the cells of ventral iris.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Eye Institute (NEI)
• Type: Intramural Research (Z01)
• Project #: 1Z01EY000311-03
• Application #: 6106863
• Study Section: Special Emphasis Panel (LMDB)
• Support Year: 3
• Fiscal Year: 1998

Institution

• Name: U.S. National Eye Institute
• Country: United States

Publications

Nakaya, Naoki; Sultana, Afia; Munasinghe, Jeeva et al. (2013) Deletion in the N-terminal half of olfactomedin 1 modifies its interaction with synaptic proteins and causes brain dystrophy and abnormal behavior in mice. Exp Neurol 250:205-18

Nakaya, Naoki; Lee, Hee-Sheung; Takada, Yuichiro et al. (2008) Zebrafish olfactomedin 1 regulates retinal axon elongation in vivo and is a modulator of Wnt signaling pathway. J Neurosci 28:7900-10

Lee, Hee-Sheung; Tomarev, Stanislav I (2007) Optimedin induces expression of N-cadherin and stimulates aggregation of NGF-stimulated PC12 cells. Exp Cell Res 313:98-108

Galeeva, A; Treuter, E; Tomarev, S et al. (2007) A prospero-related homeobox gene Prox-1 is expressed during postnatal brain development as well as in the adult rodent brain. Neuroscience 146:604-16

Nakaya, Naoki; Tomarev, Stanislav (2007) Expression patterns of alternative transcripts of the zebrafish olfactomedin 1 genes. Gene Expr Patterns 7:723-9

Wilting, Jorg; Aref, Yama; Huang, Ruijin et al. (2006) Dual origin of avian lymphatics. Dev Biol 292:165-73

Grinchuk, Oleg; Kozmik, Zbynek; Wu, Xiaofang et al. (2005) The Optimedin gene is a downstream target of Pax6. J Biol Chem 280:35228-37

Reis, Rui M; Reis-Filho, Jorge S; Longatto Filho, Adhemar et al. (2005) Differential Prox-1 and CD 31 expression in mucousae, cutaneous and soft tissue vascular lesions and tumors. Pathol Res Pract 201:771-6

Murphy, Patrick J M; Morishima, Yoshihiro; Kovacs, Jeffrey J et al. (2005) Regulation of the dynamics of hsp90 action on the glucocorticoid receptor by acetylation/deacetylation of the chaperone. J Biol Chem 280:33792-9

Steffensen, Knut R; Holter, Elin; Bavner, Ann et al. (2004) Functional conservation of interactions between a homeodomain cofactor and a mammalian FTZ-F1 homologue. EMBO Rep 5:613-9