Abstract

The proliferative potential of normal mammalian somatic cells is limited, with the vast majority daughter cells eventually entering either a senescent state or undergoing apoptotic crisis. In rodent cell cultures, the emergence of immortalized cell lineages also occurs at a readily detectable frequency. To evaluate the roles of gene products that modulate higher order chromatin structure in these growth regulatory processes, chromodomain-containing HP1 family proteins, previously linked to heterochromatin-mediated silencing, have been examined. Proteins of this class, specifically M31, M32, and HP1-alpha, were demonstrated to extend proliferative potential and protect against crisis. No significant increase in immortalization was observed. Such findings suggest a direct link between senescence and the integrity of higher- order chromatin-mediated silencing. - senescence, aging, cancer, epigenetic inheritance, chromatin, heterochromatin

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Intramural Research (Z01)
Project #
1Z01HD008719-19
Application #
6290265
Study Section
Special Emphasis Panel (LMGR)
Project Start
Project End
Budget Start
Budget End
Support Year
19
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Eunice Kennedy Shriver National Institute of Child Health & Human Development
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Humphrey, Glen W; Wang, Yong-Hong; Hirai, Tazuko et al. (2008) Complementary roles for histone deacetylases 1, 2, and 3 in differentiation of pluripotent stem cells. Differentiation 76:348-56
Golikov, Vladislav; Logacheva, Irina; Bruk, Gennadi et al. (2004) Modelling of long-term behaviour of caesium and strontium radionuclides in the Arctic environment and human exposure. J Environ Radioact 74:159-69
Prichep, L S; Gugino, L D; John, E R et al. (2004) The Patient State Index as an indicator of the level of hypnosis under general anaesthesia. Br J Anaesth 92:393-9
Oughton, Deborah; Forsberg, Ellen-Marie; Bay, Ingrid et al. (2004) An ethical dimension to sustainable restoration and long-term management of contaminated areas. J Environ Radioact 74:171-83
Bae, Nancy S; Swanson, Mark J; Vassilev, Alex et al. (2004) Human histone deacetylase SIRT2 interacts with the homeobox transcription factor HOXA10. J Biochem (Tokyo) 135:695-700
Howard, B; van Herck, H; Guillen, J et al. (2004) Report of the FELASA Working Group on evaluation of quality systems for animal units. Lab Anim 38:103-18
Russanova, Valya R; Hirai, Tazuko H; Howard, Bruce H (2004) Semirandom sampling to detect differentiation-related and age-related epigenome remodeling. J Gerontol A Biol Sci Med Sci 59:1221-33
Russanova, Valya R; Hirai, Tazuko H; Tchernov, Andrei V et al. (2004) Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach. J Gerontol A Biol Sci Med Sci 59:1234-43
Yamagoe, Satoshi; Kanno, Tomohiko; Kanno, Yuka et al. (2003) Interaction of histone acetylases and deacetylases in vivo. Mol Cell Biol 23:1025-33
Ritenbaugh, Cheryl; Patterson, Ruth E; Chlebowski, Rowan T et al. (2003) The Women's Health Initiative Dietary Modification trial: overview and baseline characteristics of participants. Ann Epidemiol 13:S87-97

Showing the most recent 10 out of 15 publications