It has been demonstrated that lysine and histidine residues in glutamine synthetase and glucose-6-phosphate dehydrogenase are among several amino acid residues that are oxidized to carbonyl derivatives by mixed-function oxidation systems. The Fenton reagent comprised of Fe-chelate and hydrogen peroxide was shown to catalyze the conversion of all common amino acids to ammonia and their corresponding Alpha-keto acid derivatives. A number of physiological chelating agents (citrate, nucleoside di- and tri- phosphates, orthophosphate, pyrophosphate) as well as nonphysiological chelating agents (EDTA, EGTA, DETAPAC, o-phenanthroline, and Alpha-dipyridyl) stimulate the oxidative deamination reaction about 2-4-fold. In addition to chelated iron, it was found that unchelated iron and also bicarbonate ion are essential for deamination to occur.
| Noda, Yasuko; Berlett, Barbara S; Stadtman, Earl R et al. (2007) Identification of enzymes and regulatory proteins in Escherichia coli that are oxidized under nitrogen, carbon, or phosphate starvation. Proc Natl Acad Sci U S A 104:18456-60 |
| Tanaka, Mikiei; Chock, P Boon; Stadtman, Earl R (2007) Oxidized messenger RNA induces translation errors. Proc Natl Acad Sci U S A 104:66-71 |
| Miyoshi, Noriyuki; Oubrahim, Hammou; Chock, P Boon et al. (2006) Age-dependent cell death and the role of ATP in hydrogen peroxide-induced apoptosis and necrosis. Proc Natl Acad Sci U S A 103:1727-31 |
| Khan, Mohammed A S; Chock, P Boon; Stadtman, Earl R (2005) Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102:17326-31 |
| Stadtman, Earl R; Van Remmen, Holly; Richardson, Arlan et al. (2005) Methionine oxidation and aging. Biochim Biophys Acta 1703:135-40 |
| Lee, Byung Cheon; Lee, Yong Kwon; Lee, Ho-Joung et al. (2005) Cloning and characterization of antioxidant enzyme methionine sulfoxide-S-reductase from Caenorhabditis elegans. Arch Biochem Biophys 434:275-81 |
| Stadtman, Earl R; Arai, Hirofumi; Berlett, Barbara S (2005) Protein oxidation by the cytochrome P450 mixed-function oxidation system. Biochem Biophys Res Commun 338:432-6 |
| Oubrahim, Hammou; Wang, Jun; Stadtman, Earl R et al. (2005) Molecular cloning and characterization of murine caspase-12 gene promoter. Proc Natl Acad Sci U S A 102:2322-7 |
| Arai, Hirofumi; Berlett, Barbara S; Chock, P Boon et al. (2005) Effect of bicarbonate on iron-mediated oxidation of low-density lipoprotein. Proc Natl Acad Sci U S A 102:10472-7 |
| Williams, W M; Stadtman, E R; Moskovitz, J (2004) Ageing and exposure to oxidative stress in vivo differentially affect cellular levels of PrP in mouse cerebral microvessels and brain parenchyma. Neuropathol Appl Neurobiol 30:161-8 |
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