Structure of Natural Products Using Instrumental Methods

Fales, H M.

Abstract

A variety of natural products have been studied using GC-MS, NMR and HPLC, including the granary weevil pheromone, 3 new amino acids from S. lactis, a vitamin E-dimer, and numerous insect constituents. Experiments on the possible biosynthesis of valium have been initiated and several studies using the Cf-252 spectrometer have been successful.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HL001003-17
• Application #: 3942806
• Support Year: 17
• Fiscal Year: 1987

Institution

• Name: U.S. National Heart Lung and Blood Inst
• Country: United States

Publications

Lindh, Rebecka; Ahmad, Faiyaz; Resjo, Svante et al. (2007) Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B. Biochim Biophys Acta 1773:584-92

Ludwig, Joseph A; Szakacs, Gergely; Martin, Scott E et al. (2006) Selective toxicity of NSC73306 in MDR1-positive cells as a new strategy to circumvent multidrug resistance in cancer. Cancer Res 66:4808-15

Davis, David A; Singer, Kathleen E; De La Luz Sierra, Maria et al. (2005) Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1alpha in the circulation. Blood 105:4561-8

Goren, Mayer B; Sokoloski, Edward A; Fales, Henry M (2005) 2-Methyl-(1Z,3E)-butadiene-1,3,4-tricarboxylic acid, ""isoprenetricarboxylic acid"". J Org Chem 70:7429-31

Boja, Emily S; Hoodbhoy, Tanya; Garfield, Mark et al. (2005) Structural conservation of mouse and rat zona pellucida glycoproteins. Probing the native rat zona pellucida proteome by mass spectrometry. Biochemistry 44:16445-60

Zhao, Hongtao; Joseph, Joy; Fales, Henry M et al. (2005) Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A 102:5727-32

Che, Magnus M; Boja, Emily S; Yoon, Hye-Young et al. (2005) Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains. Cell Signal 17:1276-88

Boja, Emily S; Sokoloski, Edward A; Fales, Henry M (2004) Divinyl sulfone as a postdigestion modifier for enhancing the a(1) Ion in MS/MS and postsource decay: potential applications in proteomics. Anal Chem 76:3958-70

Joubert, Frederic; Fales, Henry M; Wen, Han et al. (2004) NADH enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP): applications to enzyme and mitochondrial reaction kinetics, in vitro. Biophys J 86:629-45

Sun, G; Ma, Y; Gao, X et al. (2004) Method for isolating tight-binding inhibitors of rat lens aldose reductase. Exp Eye Res 79:919-26