Analysis Of Peptides, Proteins, And Metabolites Using Ma

Fales, Henry M.

Abstract

This year we have again been involved in many projects relating to the analysis of peptides and proteins brought to our attention by researchers at NHLBI and NIH. Among many others, these include the development of new bridging agents to determine protein structure using mass spectrometry, determination of certain sites overalkylated with iodoacetamide, determination of a phosphorylation site in recombinant PKC, evaluation of a drug-resistant HIV protease-C95F mutation, elucidation of the structure of a putative endogenous aldose reductase inhibitor, identified a fire-blight compound as the known dihomoserine isourea, identified structures of reaction products of KNI-272 a putative anti-HIV drug,determined phosphorylation sites of IRS1 by PKC-zeta, identified several proteins from 1D and 2D gels including DNA-binding protein, GB3 colocalized proteins in colon cancer and proteins coprecipitated with NHE3 in intestinal cancer, determined glutathionylation of actin under oxidative stress, evaluated ICAT methods on BSA and laminin, performed de-novo sequencing of a novel protein in amoeba, determined phosphorylation sites in PAK, PLC-beta-1 and gamma 2.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Intramural Research (Z01)
• Project #: 1Z01HL001003-29
• Application #: 6541675
• Study Section: (LBC)
• Support Year: 29
• Fiscal Year: 2001

Institution

• Name: U.S. National Heart Lung and Blood Inst
• Country: United States

Publications

Lindh, Rebecka; Ahmad, Faiyaz; Resjo, Svante et al. (2007) Multisite phosphorylation of adipocyte and hepatocyte phosphodiesterase 3B. Biochim Biophys Acta 1773:584-92

Ludwig, Joseph A; Szakacs, Gergely; Martin, Scott E et al. (2006) Selective toxicity of NSC73306 in MDR1-positive cells as a new strategy to circumvent multidrug resistance in cancer. Cancer Res 66:4808-15

Davis, David A; Singer, Kathleen E; De La Luz Sierra, Maria et al. (2005) Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1alpha in the circulation. Blood 105:4561-8

Goren, Mayer B; Sokoloski, Edward A; Fales, Henry M (2005) 2-Methyl-(1Z,3E)-butadiene-1,3,4-tricarboxylic acid, ""isoprenetricarboxylic acid"". J Org Chem 70:7429-31

Boja, Emily S; Hoodbhoy, Tanya; Garfield, Mark et al. (2005) Structural conservation of mouse and rat zona pellucida glycoproteins. Probing the native rat zona pellucida proteome by mass spectrometry. Biochemistry 44:16445-60

Zhao, Hongtao; Joseph, Joy; Fales, Henry M et al. (2005) Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and limitations of fluorescence. Proc Natl Acad Sci U S A 102:5727-32

Che, Magnus M; Boja, Emily S; Yoon, Hye-Young et al. (2005) Regulation of ASAP1 by phospholipids is dependent on the interface between the PH and Arf GAP domains. Cell Signal 17:1276-88

Sun, G; Ma, Y; Gao, X et al. (2004) Method for isolating tight-binding inhibitors of rat lens aldose reductase. Exp Eye Res 79:919-26

De La Luz Sierra, Maria; Yang, Fuquan; Narazaki, Masashi et al. (2004) Differential processing of stromal-derived factor-1alpha and stromal-derived factor-1beta explains functional diversity. Blood 103:2452-9

Jeong, Woojin; Chang, Tong-Shin; Boja, Emily S et al. (2004) Roles of TRP14, a thioredoxin-related protein in tumor necrosis factor-alpha signaling pathways. J Biol Chem 279:3151-9