Abstract

Solid State and solution nuclear magnetic resonance spectroscopy has been used to study the structure of peptides in various noncrystalline states which may be similar to the physiological environment. The peptides we have studied include a series of peptides which is a part of the integral membrane protein, gp4l, found at the surface of human immunodeficiency virus. As a result of these conformational studies we have identified transmembrane regions as well as other regions which interact exclusively with the phosphate polar headgroups of the membrane bilayer. We have also studied the solution structure of two peptides associated with a region of the gp4l. One peptide is highly antigenic and distinguishes between the sera of healthy HIV positive patients from those with AIDS. The corresponding peptide where alanine is replaced by threonine in position seven does not show such a distinction. We have also developed new NMR pulse methods for studying these systems and we are continuing to evaluate the errors in the corresponding measurements. We have developed and implemented new NMR pulse methods for studying these systems and we are continuing to evaluate the errors in the measurements.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL001027-08
Application #
3878924
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Wu, Xiongwu; Pickard 4th, Frank C; Brooks, Bernard R (2016) Isotropic periodic sum for multipole interactions and a vector relation for calculation of the Cartesian multipole tensor. J Chem Phys 145:164110
Rinschen, Markus M; Bharill, Puneet; Wu, Xiongwu et al. (2016) The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes. Hum Mol Genet 25:1328-44
Roubinian, Nareg H; Looney, Mark R; Kor, Daryl J et al. (2015) Cytokines and clinical predictors in distinguishing pulmonary transfusion reactions. Transfusion 55:1838-46
Wu, Xiongwu; Brooks, Bernard R (2015) A Virtual Mixture Approach to the Study of Multistate Equilibrium: Application to Constant pH Simulation in Explicit Water. PLoS Comput Biol 11:e1004480
Wu, Xiongwu; Subramaniam, Sriram; Case, David A et al. (2013) Targeted conformational search with map-restrained self-guided Langevin dynamics: application to flexible fitting into electron microscopic density maps. J Struct Biol 183:429-440
Singh, Rishi P; Brooks, Bernard R; Klauda, Jeffery B (2009) Binding and release of cholesterol in the Osh4 protein of yeast. Proteins 75:468-77
Lengyel, Jeffrey S; Stott, Katherine M; Wu, Xiongwu et al. (2008) Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex. Structure 16:93-103
Hwang, Kae-Jung; Mahmoodian, Fatemeh; Ferretti, James A et al. (2007) Intramolecular interaction in the tail of Acanthamoeba myosin IC between the SH3 domain and a putative pleckstrin homology domain. Proc Natl Acad Sci U S A 104:784-9
Ju, Jeong Ho; Maeng, Jin-Soo; Zemedkun, Micheas et al. (2006) Physical and functional interactions between the prostate suppressor homeoprotein NKX3.1 and serum response factor. J Mol Biol 360:989-99
Milne, Jacqueline L S; Wu, Xiongwu; Borgnia, Mario J et al. (2006) Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy. J Biol Chem 281:4364-70

Showing the most recent 10 out of 26 publications