Abstract

Solution and solid state nuclear magnetic resonance spectroscopy are being used along with circular dichroism, microelectrophoretic mobility, and fluorescence measurements to study the structure of peptides and small proteins in solution and in the bound state. The peptides that have been investigated include fragments of the integral membrane envelope glycoprotein, gp4l, of human immuno-deficiency virus-1. One highly conserved fragment (828-848), which is in the cytoplasmic tail region of the gp4l, is required for infectivity. We have shown that this fragment binds electrostatically to the surface of model membranes and that it may tether the protein to the cytoplasmic surface of the viral membrane. We have also studied the structure of peptides bound to a monoclonal antibody that represents immunogenic regions of the viral coat protein of Rift Valley Fever Virus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL001027-09
Application #
3857999
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Wu, Xiongwu; Pickard 4th, Frank C; Brooks, Bernard R (2016) Isotropic periodic sum for multipole interactions and a vector relation for calculation of the Cartesian multipole tensor. J Chem Phys 145:164110
Rinschen, Markus M; Bharill, Puneet; Wu, Xiongwu et al. (2016) The ubiquitin ligase Ubr4 controls stability of podocin/MEC-2 supercomplexes. Hum Mol Genet 25:1328-44
Roubinian, Nareg H; Looney, Mark R; Kor, Daryl J et al. (2015) Cytokines and clinical predictors in distinguishing pulmonary transfusion reactions. Transfusion 55:1838-46
Wu, Xiongwu; Brooks, Bernard R (2015) A Virtual Mixture Approach to the Study of Multistate Equilibrium: Application to Constant pH Simulation in Explicit Water. PLoS Comput Biol 11:e1004480
Wu, Xiongwu; Subramaniam, Sriram; Case, David A et al. (2013) Targeted conformational search with map-restrained self-guided Langevin dynamics: application to flexible fitting into electron microscopic density maps. J Struct Biol 183:429-440
Singh, Rishi P; Brooks, Bernard R; Klauda, Jeffery B (2009) Binding and release of cholesterol in the Osh4 protein of yeast. Proteins 75:468-77
Lengyel, Jeffrey S; Stott, Katherine M; Wu, Xiongwu et al. (2008) Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex. Structure 16:93-103
Hwang, Kae-Jung; Mahmoodian, Fatemeh; Ferretti, James A et al. (2007) Intramolecular interaction in the tail of Acanthamoeba myosin IC between the SH3 domain and a putative pleckstrin homology domain. Proc Natl Acad Sci U S A 104:784-9
Ju, Jeong Ho; Maeng, Jin-Soo; Zemedkun, Micheas et al. (2006) Physical and functional interactions between the prostate suppressor homeoprotein NKX3.1 and serum response factor. J Mol Biol 360:989-99
Milne, Jacqueline L S; Wu, Xiongwu; Borgnia, Mario J et al. (2006) Molecular structure of a 9-MDa icosahedral pyruvate dehydrogenase subcomplex containing the E2 and E3 enzymes using cryoelectron microscopy. J Biol Chem 281:4364-70

Showing the most recent 10 out of 26 publications