Large quantities of salt and water move across epithelial cells. These cells are able to maintain a constant volume by balancing solute entry and exit. The mechanisms for epithelial cell volume regulation are under investigation in this laboratory. Optical and microelectrode studies have been performed on the gallbladder of Necturus, on the renal cortical collecting tubule of the rabbit, the toad skin, malpighian tubules, on the renal papillary epithelium, and on mosquito.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL001266-04
Application #
3966568
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1986
Total Cost
Indirect Cost
Name
U.S. National Heart Lung and Blood Inst
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Praetorius, Jeppe; Spring, Kenneth R (2002) Specific lectins map the distribution of fibronectin and beta 1-integrin on living MDCK cells. Exp Cell Res 276:52-62
Praetorius, H A; Spring, K R (2001) Bending the MDCK cell primary cilium increases intracellular calcium. J Membr Biol 184:71-9
Kovbasnjuk, O N; Spring, K R (2000) The apical membrane glycocalyx of MDCK cells. J Membr Biol 176:19-29
Spring, K R (2000) Scientific imaging with digital cameras. Biotechniques 29:70-2, 74, 76
Kovbasnjuk, O N; Bungay, P M; Spring, K R (2000) Diffusion of small solutes in the lateral intercellular spaces of MDCK cell epithelium grown on permeable supports. J Membr Biol 175:16-Sep