The mechanism of fluid transport by epithelial tissues such as kidney cells has been investigated by fluorescence microscopy of living cultured renal cells using methods developed in this laboratory. These studies have produced a detailed understanding of the mechanism of fluid transport. Recently, we have shown that charged sugar moieties of the glycocalyx on the epithelial cell surface play an important role in the controlling the near-membrane pH and microenvironment. - fluid transport, kidney, MDCK cells, glycocalyx
| Praetorius, Jeppe; Spring, Kenneth R (2002) Specific lectins map the distribution of fibronectin and beta 1-integrin on living MDCK cells. Exp Cell Res 276:52-62 |
| Praetorius, H A; Spring, K R (2001) Bending the MDCK cell primary cilium increases intracellular calcium. J Membr Biol 184:71-9 |
| Kovbasnjuk, O N; Spring, K R (2000) The apical membrane glycocalyx of MDCK cells. J Membr Biol 176:19-29 |
| Spring, K R (2000) Scientific imaging with digital cameras. Biotechniques 29:70-2, 74, 76 |
| Kovbasnjuk, O N; Bungay, P M; Spring, K R (2000) Diffusion of small solutes in the lateral intercellular spaces of MDCK cell epithelium grown on permeable supports. J Membr Biol 175:16-Sep |
