The kidney contains several distinct epithelia that, in their aggregate function, are responsible for formation of the urine and the maintenance of fluid and electrolyte homeostasis. Each epithelium has a unique population of sodium and chloride ion transporters and urea transporters, as well as water channels (aquaporins, which in aggregate regulate NaCl and water excretion. Understanding the regulation of these transporters is a key to the ultimate understanding of the pathophysiology of essential hypertension, salt retention in congestive heart failure and disorders of water balance. We are investigating these epithelia and their transporters using a combination of methodologies based on proteomics and oligonucleotide array technologies and the development of transgenic mice, as well as classical immunochemical and transport measurements in isolated perfused tubules. The current focus is on: 1) mechanisms of NaCl retention in hypertension and in extracellular fluid volume expanded states (congestive heart failure, cirrhosis, nephrotic syndrome); 2) the molecular physiology of the urinary concentrating mechanism with emphasis on the role of urea transporters and aquaporins; 3)development and characterization of polyclonal antibodies against renal transporter and channel proteins; 4) molecular physiology of renal NaCl transport regulation including regulation of the epithelial sodium channel (ENaC) by vasopressin and aldosterone; 5) mathematical modeling of renal transport and signaling processes.
| Saethang, Thammakorn; Hodge, Kenneth; Kimkong, Ingorn et al. (2018) AbDesigner3D: a structure-guided tool for peptide-based antibody production. Bioinformatics 34:2158-2160 |
| LeMaire, Sophia M; Raghuram, Viswanathan; Grady, Cameron R et al. (2017) Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct. Am J Physiol Renal Physiol 312:F84-F95 |
| Sandoval, Pablo C; Claxton, J'Neka S; Lee, Jae Wook et al. (2016) Systems-level analysis reveals selective regulation of Aqp2 gene expression by vasopressin. Sci Rep 6:34863 |
| Cheng, Lei; Pisitkun, Trairak; Knepper, Mark A et al. (2016) Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics. Methods Mol Biol 1355:53-70 |
| Saeed, Fahad; Hoffert, Jason D; Knepper, Mark A (2014) CAMS-RS: Clustering Algorithm for Large-Scale Mass Spectrometry Data Using Restricted Search Space and Intelligent Random Sampling. IEEE/ACM Trans Comput Biol Bioinform 11:128-41 |
| Pisitkun, Trairak; Dummer, Patrick; Somparn, Poorichaya et al. (2014) Integrated Design of Antibodies for Systems Biology Using Ab Designer. J Proteomics Bioinform 7:088-94 |
| Knepper, Mark A (2014) Proteomic pearl diving versus systems biology in cell physiology. Focus on ""proteomic mapping of proteins released during necrosis and apoptosis from cultured neonatal cardiac myocytes"". Am J Physiol Cell Physiol 306:C634-5 |
| Miranda, Carlos A; Lee, Jae Wook; Chou, Chung-Lin et al. (2014) Tolvaptan as a tool in renal physiology. Am J Physiol Renal Physiol 306:F359-66 |
| Sanghi, Akshay; Zaringhalam, Matthew; Corcoran, Callan C et al. (2014) A knowledge base of vasopressin actions in the kidney. Am J Physiol Renal Physiol 307:F747-55 |
| Bradford, Davis; Raghuram, Viswanathan; Wilson, Justin L L et al. (2014) Use of LC-MS/MS and Bayes' theorem to identify protein kinases that phosphorylate aquaporin-2 at Ser256. Am J Physiol Cell Physiol 307:C123-39 |
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