Results from numerous in vivo studies have suggested that a new dopamine agonist, B-HT 920, may be selective for stimulation of dopamine autoreceptors. To examine this question, the inhibition of K+stimulated [3H] dopamine and [14C]acetycholine release was used as an in vitro measure of presynaptic and postsynaptic dopamine receptor stimulation, respectively. In contrast to the results of in vivo studies, B-HT 920 was found to be a potent agonist at both pre- and postsynaptic dopamine recetors. Both effects of B-HT 920 were stereospecific and antagnoized by the D2 dopamine antagonist, sulpiride, while the alpha antagonist, tolazoline, was without effect. The presynaptic effect of B-HT 920 was selective to dopamine neurons in that K+-stimulated [3H]serotonin release was not affected.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL003547-01
Application #
4694638
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
U.S. National Heart Lung and Blood Inst
Department
Type
DUNS #
City
State
Country
United States
Zip Code