We have previously shown that patients with essential hypertension have impaired endothelium-dependent vascular relaxation. In a separate investigation, we demonstrated that increased availability of precursor for endothelium-derived nitric oxide is rate-limiting and can potentiate the response to endothelium-dependent vasodilators in normal humans. The present study was undertaken to determine whether the abnormal endothelium-dependent vascular relaxation of hypertensive patients can be modified by increasing availability of nitric oxide substrate. To this purpose, 14 hypertensive patients (age 48 +/- 7 years; 9 men and 5 women) were studied with intra-arterial infusion of acetylcholine (endothelium-dependent vasodilator) and sodium nitroprusside (a direct smooth muscle vasodilator) before and after the infusion of L-Arginine, the natural substrate for production of endothelium-derived nitric oxide. The results were compared with those obtained in a control group of 12 normal volunteers (age 49 +/- 7 years; 7 men and 5 women) matched to the patients for age and sex. As shown previously, the vasodilator response to acetylcholine was blunted in hypertensive patients compared to controls; however, no difference was observed in the response to sodium nitroprusside. The infusion of L-Arginine did not produce any significant change in basal blood flow in either hypertensive patients or normal controls. In contrast to normal controls, the infusion of L- Arginine did not modify the vasodilator response to acetylcholine. Similarly, the response to sodium nitroprusside was not altered by L- Arginine. These findings indicate that the abnormal endothelium- dependent vasodilation of hypertensive patients is not due to decreased nitric oxide substrate availability, and further suggest a defect in the synthesis or release of nitric oxide by endothelial cells.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Intramural Research (Z01)
Project #
1Z01HL004918-01
Application #
3843418
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1992
Total Cost
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code