Branches, labs and projects supported? ? Flow Cytometry Core-- work done, no project title given? ? Hematology Branch ? 1. Neal Young-- Confocal imaging of bonemarrow tissue? 2. Robert Donahue-- work done, no project title given? 3. Elaine Sloand-- Determination of mechanism for aneuploidy in patients with TERT and TERC mutations: examination of telomere fusion by confocal microscopy? ? Lab of Biochemistry (Boon Chock)work done, no project title given? ? Lab of Biochemical Genetics ? 1. Marshall Nirenberg? a. Transcriptional regulatory networks directing myoblast gene expression in Drosophila embryos ? b. RNA interference screen of the genes involved in drosophila neurogenesis? c. Functional analysis of the upstream region of the nk-2/vnd gene? 2. Robert Kotin? a. Imaging AAV distribution and transgene GFP expression in vivo ? b. Detecting cell morphology and observation GFP expression after transduction AAV-shHec1 vector? ? Lab of Cardiac Energetics (Robert Balaban)collaborations involving multi-photon microscopy,Total Emission Detection System for Multi-photon Imaging, FLIM NADH imaging, and CARS water imaging pilot project ? ? Lab of Cell Biology (Rosa Puertollano-Moro)-- work done, no project title given? ? Lab of Developmental Biology? Cecilia LoThe role of a novel gene Megf8 in left-right determination? Mukoyama, Yosuke-- work done, no project title given ? Ken Kramer-- work done, no project title given? ? ? ? Lab of Developmental Systems Biology? Alan Michelson-- work done, no project title given? ? Lab of Kidney and Electrolyte Metabolism? 1. Mark Knepper-- Vasopressin action in the kidney? 2. Raghuram, Viswanathan-- work done, no project title given ? ? 3. Maurice Berg? a. Tonicity dependent nucleocytoplasmic trafficking of TonEBP? b. Coactivator and regulation of TonEBP activation? c. GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte glycerophosphocholine? d. Studies of DNA damage induced by high NaCl? e. Analysis of renal function and structure of Ku86-/- mice? ? Lab of Molecular Biophysics? 1. Nico Tjandra (will begin work on new project in July 2008 in core, no title given)? 2. Kier Neuman (used custom written software in core for image/data analysis)? a. DNA unwinding of RecQ Helicase ? b. Movement of Collagenase on Collagen Fibers? 3. Jennifer Lee (donated equipment to her lab)? 4. Jay Knutson? a. FLIM and photobleached FRET on dendritic cells for cancer immunotherapy? b. Total Emission Detection System for Multi-photon Imaging? ? Lab of Molecular Cardiology ( Bob Adelstein)? a. The Role of Nonmuscle Myosins in Development ? b. The Role of Nonmuscle Myosin II-B in Mouse Heart and Brain Development ? c. Alternative Splicing of Nonmuscle Myosin Heavy Chains? d. The Function of Nonmuscle Myosin Heavy Chains: Using Homologous ? e. Recombination to Replace Nonmuscle Myosin II-A with Human GFP-Myosin II-B ? in Mice? f. In Vivo Function of Nonmuscle Myosin II-A? ? Lab of Molecular Immunology? Warren Leonardwork done, no project title given? Michael Beaven-- Effects of PLD in lipid raft formation? ? Lab of Molecular Physiology? James Sellers? a. Movement of myosin on higher order actin structures ? b. Role of myosin V in Drosophila embryos? ? Lipid Trafficking Corework done, no project title given? ? Office of Education (Herb Geller)? a. Role of myosin II in growth cone turning at the boundary with chondroitin sulfate proteoglycans? b. RhoA activation in response to chondroitin sulfate proteoglycans? c. 4-sulfated proteoglycans are major inhibitors of axonal growth in the injured spinal cord? Pulmonary and Vascular Medicine Branch? 1. Mark Gladwin--- ? a. Nitrite effects in a mouse model of cardiac arrest? b. Circulating blood cell versus endothelial cell contribtions to NOS-mediated regulation of blood flow in murine skeletal muscle? 2. Alan Remaly and Marcelo Amar? a. Development of imaging methods for quantification of body fat in mice (protocol #H0116)? b. Development of methods for analysis of atherosclerotic plaque formation in mouse carotid arteries (protocol H-0050R1)? c. Imaging of fluorescent lipoprotein uptake into plaques of mice with ligated carotid arteries (protocol #H0116)? 3. Elizabeth Murphy (work done, no project title provided)? ? ? Transgenic Core-- Characterization of the defects that cause dwarfism and obesity in TTC3 knockout mice? ? Translational Medicine Branch? 1. Toren Finkel ? a. Wnt signaling and mammalian aging ? b. Regulation of autophagy? ? 2. Elizabeth Nabelwork done, no project title given? 3. Howard Kruth? a. Function of Src Family Kinases in Macrophage Foam Cell Formation? b. Function of UBIAD1 in Schnyder Cholesterol Corneal Dystrophy? 4. Andrew Arai-- Late Gadolinium Enhancement Identifies Post Infarction Myocardial Fibrosis and the Border Zone at the Cellular Level in ex vivo Rat Heart? 5. Manfred Boehm? a. the application of murine embryonic stem cells on mouse hindlimb ischemic model? b. Murine Model of Vein Grafting (H-0184)? c. Cardiovascular lineage differentiation of embryonic stem cells? d. Lineage tracking the cell-mediated repair response in murine myocardial infarction? e. p21 modulates arterial wound repair through the SDF-1/CXCR4 axis? ? 6. Paul Hwang-- work done, no project title given? 7. Vince Manganiello---work done, no project title given? 8. Joel Moss? a. Erythropoietin-driven signal transduction in Lymphangioleiomyomatosis ? b. Chemokine-enhanced chemotaxis of LAM cells with mutations in the tumor ? suppressor TSC2 gene? c. Intracellular actions of VEGF-D in human lung fibroblasts? 9. Stew Levine-- cAMP-dependent PKA Signaling Induces TNFR1 Exosome-like Vesicle Release via Anchoring of PKA Regulatory Subunit RIIb to BIG2? 10. Martha Vaughn-- Molecular Characterization and Regulation of GTP-binding Proteins? ? 11. Don Orlic? a. Stem cell regeneration of vascular tissue in eNOS-/- mice? b. Stem cell regeneration of the vascular system in Progeria mice? ? 12. Han Wen-- Spatial harmonic imaging of x-ray scattering? ? ? Dr. Rogers (Clinical Hematology)- Functional study of hGC-1 gene in prostate cancer cells and ? knock-out mouse model? ? Clinical Center Researchers? a. Establishing CD36 and CLA-1 as Novel Adenoviral Receptors Mediating Viral ? infectivity ? 2) Cytotoxicity in the Liver ? 3) avB3-targeted imaging of early atherosclerosis in Watanabe Heritable Hyperlipidemia (WHHL) rabbits with a new integrin antagonist ? ? ? Other Institutes--? ? NIBIB and NEI (Dr.s Amaral and Lutz)-- Efficacy of Pigment Epithelium-derived Factor (PEDF) ? on laser-Induced Choroidal Neovascularization (CNV) using a Subconjunctival Thermosensitive Gel Delivery System
