Abstract

The goals of this project are to study mechanisms underlying the developmental and homeostatic regulation of the gene expression, posttranslational modification, and secretion of neuropeptides with a focus on the magnocellular oxytocin and vasopressin neurons in the hypothalamus. Efforts are being made to develop in vitro (dissociated cell and slice explant cultures) and in vivo (transgenic mouse) model systems to study these issues. Preliminary results indicate that both types of model systems are close to being achieved. The approach is cell- biological and hence, the focus is on the activity and gene expression at the single cell level. For this purpose, immunocytochemical, in situ hybridization histochemical, and single cell mRNA techniques are utilized.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Intramural Research (Z01)
Project #
1Z01NS002723-08
Application #
3760270
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
National Institute of Neurological Disorders and Stroke
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Rusnak, Milan; Toth, Zsuzsanna E; House, Shirley B et al. (2007) Depolarization and neurotransmitter regulation of vasopressin gene expression in the rat suprachiasmatic nucleus in vitro. J Neurosci 27:141-51
Ponzio, Todd A; Yue, Chunmei; Gainer, Harold (2007) An intron-based real-time PCR method for measuring vasopressin gene transcription. J Neurosci Methods 164:149-54
House, Shirley B; Rusnak, Milan; Liu, Xiu-Huai et al. (2006) Bcl-xL and caspase inhibition increase the survival of rat oxytocin and vasopressin magnocellular neurons in organotypic culture. Exp Neurol 200:267-71
Mutsuga, Noriko; Gainer, Harold (2006) Molecular analysis of the magnocellular neuroendocrine phenotype: from the micropunch to laser microdissection. Neurochem Res 31:189-99
Yue, Chunmei; Mutsuga, Noriko; Scordalakes, Elka M et al. (2006) Studies of oxytocin and vasopressin gene expression in the rat hypothalamus using exon- and intron-specific probes. Am J Physiol Regul Integr Comp Physiol 290:R1233-41
Yue, Chunmei; Mutsuga, Noriko; Verbalis, Joseph et al. (2006) Microarray analysis of gene expression in the supraoptic nucleus of normoosmotic and hypoosmotic rats. Cell Mol Neurobiol 26:959-78
Glasgow, Eric; Ryu, Seung-Lim; Yamashita, Mitsuo et al. (2005) APeg3, a novel paternally expressed gene 3 antisense RNA transcript specifically expressed in vasopressinergic magnocellular neurons in the rat supraoptic nucleus. Brain Res Mol Brain Res 137:143-51
Rusnak, Milan; Gainer, Harold (2005) Differential effects of forskolin on tyrosine hydroxylase gene transcription in identified brainstem catecholaminergic neuronal subtypes in organotypic culture. Eur J Neurosci 21:889-98
Zhang, Bing-Jun; Yamashita, Mitsuo; Fields, Ray et al. (2005) EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells. Cell Mol Neurobiol 25:581-605
Mutsuga, Noriko; Shahar, Tal; Verbalis, Joseph G et al. (2005) Regulation of gene expression in magnocellular neurons in rat supraoptic nucleus during sustained hypoosmolality. Endocrinology 146:1254-67

Showing the most recent 10 out of 33 publications