In response to external and internal signals, mammalian cells elicit changes in gene expression patterns that profoundly influence the global cellular response. The transcriptional events that regulate gene expression changes have been thoroughly studied, but less-well understood post-transcriptional processes are emerging as major regulatory mechanisms. Post-transcriptional gene regulation includes pre-mRNA processing and maturation, mRNA transport, stability and translation, as well as protein processing, modification and degradation. We are keenly interested in the mechanisms that regulate the expression of proliferative, cell cycle-regulatory, and stress-response proteins. Over the past 10 years, this Project has examined mRNAs encoding various stress-response and proliferative proteins. During this funding period, we have focused our attention on mRNAs encoding heme oxygenase (HO)-1 and c-myc. We have reported that RBP associates with HO-1 mRNA and increases its stability in response to treatment with nitric oxide. We also reported that expression of the proto-oncogene and transcription factor c-myc is repressed by HuR through a mechanism that involves the recruitment of let-7 onto the c-Myc mRNA. Ongoing studies are examining the roles of RNA-binding proteins and microRNAs in the regulation of egr-1 expression in response to DNA-damaging agents, the expression of TOP2A, and the levels of HIF-1alpha after treatment with cobalt chloride and PX-478.
| Panda, Amaresh C; Gorospe, Myriam (2018) Detection and Analysis of Circular RNAs by RT-PCR. Bio Protoc 8: |
| Chung, Hee Kyoung; Wang, Shelley R; Xiao, Lan et al. (2018) ?4 Coordinates Small Intestinal Epithelium Homeostasis by Regulating Stability of HuR. Mol Cell Biol 38: |
| Xiao, Lan; Wu, Jing; Wang, Jun-Yao et al. (2018) Long Noncoding RNA uc.173 Promotes Renewal of the Intestinal Mucosa by Inducing Degradation of MicroRNA 195. Gastroenterology 154:599-611 |
| Noh, Ji Heon; Gorospe, Myriam (2018) AKTions by Cytoplasmic lncRNA CASC9 Promote Hepatocellular Carcinoma Survival. Hepatology 68:1675-1677 |
| Wang, Jun-Yao; Cui, Yu-Hong; Xiao, Lan et al. (2018) Regulation of Intestinal Epithelial Barrier Function by Long Noncoding RNA uc.173 through Interaction with MicroRNA 29b. Mol Cell Biol 38: |
| Panda, Amaresh C; Dudekula, Dawood B; Abdelmohsen, Kotb et al. (2018) Analysis of Circular RNAs Using the Web Tool CircInteractome. Methods Mol Biol 1724:43-56 |
| Cui, Chang-Yi; Noh, Ji Heon; Michel, Marc et al. (2018) STIM1, but not STIM2, Is the Calcium Sensor Critical for Sweat Secretion. J Invest Dermatol 138:704-707 |
| Noh, Ji Heon; Kim, Kyoung Mi; McClusky, Waverly G et al. (2018) Cytoplasmic functions of long noncoding RNAs. Wiley Interdiscip Rev RNA 9:e1471 |
| Basu, Sandip K; Gonit, Mesfin; Salotti, Jacqueline et al. (2018) A RAS-CaMKK?-AMPK?2 pathway promotes senescence by licensing post-translational activation of C/EBP? through a novel 3'UTR mechanism. Oncogene 37:3528-3548 |
| Kim, Kyoung Mi; Abdelmohsen, Kotb; Mustapic, Maja et al. (2017) RNA in extracellular vesicles. Wiley Interdiscip Rev RNA 8: |
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