Abstract

(1) We have continued our longitudinal pediatric cohort of dengue and Aedes aegypti seroprevalence along with active surveillance of the 2019 dengue outbreak at our field site in Kampong Speu. (2)Preliminary evidence from baseline cohort samples has identified 12 possible protein candidates. Recombinant proteins from the LMVR battery will be produced for testing against the cohort. (3)We have started the first next generation metagenomic pathogen sequencing study in Cambodia using a cloud-based platform IDseq to identify pathogens causing fevers in the community. (4) We are collecting other vectors (fleas, ticks, other mosquito species) in order to explore exposure in relation to vector-borne disease identified by sequencing. (5) We have continued entomology and geospatial studies to describe the epidemiology of Aedes-transmitted infections, and to establish and characterize spatial relationships between population abundance of Ae. aegypti, magnitude of human antibody response to Ae. aegypti saliva, and disease development.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001066-12
Application #
10014155
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Amato, Roberto; Pearson, Richard D; Almagro-Garcia, Jacob et al. (2018) Origins of the current outbreak of multidrug-resistant malaria in southeast Asia: a retrospective genetic study. Lancet Infect Dis 18:337-345
Bopp, Selina; Magistrado, Pamela; Wong, Wesley et al. (2018) Plasmepsin II-III copy number accounts for bimodal piperaquine resistance among Cambodian Plasmodium falciparum. Nat Commun 9:1769
Mukherjee, Angana; Bopp, Selina; Magistrado, Pamela et al. (2017) Artemisinin resistance without pfkelch13 mutations in Plasmodium falciparum isolates from Cambodia. Malar J 16:195
Ataide, Ricardo; Ashley, Elizabeth A; Powell, Rosanna et al. (2017) Host immunity to Plasmodium falciparum and the assessment of emerging artemisinin resistance in a multinational cohort. Proc Natl Acad Sci U S A 114:3515-3520
Amato, Roberto; Lim, Pharath; Miotto, Olivo et al. (2017) Genetic markers associated with dihydroartemisinin-piperaquine failure in Plasmodium falciparum malaria in Cambodia: a genotype-phenotype association study. Lancet Infect Dis 17:164-173
Srimuang, Krongkan; Miotto, Olivo; Lim, Pharath et al. (2016) Analysis of anti-malarial resistance markers in pfmdr1 and pfcrt across Southeast Asia in the Tracking Resistance to Artemisinin Collaboration. Malar J 15:541
Hostetler, Jessica B; Lo, Eugenia; Kanjee, Usheer et al. (2016) Independent Origin and Global Distribution of Distinct Plasmodium vivax Duffy Binding Protein Gene Duplications. PLoS Negl Trop Dis 10:e0005091
St Laurent, Brandyce; Oy, Kolthida; Miller, Becky et al. (2016) Cow-baited tents are highly effective in sampling diverse Anopheles malaria vectors in Cambodia. Malar J 15:440
Fairhurst, Rick M; Dondorp, Arjen M (2016) Artemisinin-Resistant Plasmodium falciparum Malaria. Microbiol Spectr 4:
Pearson, Richard D; Amato, Roberto; Auburn, Sarah et al. (2016) Genomic analysis of local variation and recent evolution in Plasmodium vivax. Nat Genet 48:959-64

Showing the most recent 10 out of 48 publications