Development Of Flow Cytometric Technologies For Immune Assays

Roederer, Mario

Abstract

Flow Cytometry is the primary tool in most immunology laboratories for the detailed characterization of lymphocytes and lymphocyte function. The ability to resolve highly complex populations of cells, interrogate the cells one-by-one, make multiple measurements on each cell, and then separate the cells viably for further study make this technology unique and indispensable. Our laboratory has long been involved in pushing this technology forward; we have perhaps the most sophisticated flow cytometer in the world, currently capable of making 20 different measurements on each cell. This platform has proven invaluable in dissecting immune responses to vaccines and viruses. In addition, we are developing novel algorithms for the automated analysis of the highly complex data that results from these experiments.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Investigator-Initiated Intramural Research Projects (ZIA)
• Project #: 1ZIAAI005020-14
• Application #: 9161750
• Support Year: 14
• Fiscal Year: 2015

Institution

• Name: Niaid Extramural Activities

Publications

Zurawski, Gerard; Zurawski, Sandra; Flamar, Anne-Laure et al. (2016) Targeting HIV-1 Env gp140 to LOX-1 Elicits Immune Responses in Rhesus Macaques. PLoS One 11:e0153484

Fletez-Brant, Kipper; Špidlen, Josef; Brinkman, Ryan R et al. (2016) flowClean: Automated identification and removal of fluorescence anomalies in flow cytometry data. Cytometry A 89:461-71

Roederer, Mario (2016) Distributions of autofluorescence after compensation: Be panglossian, fret not. Cytometry A 89:398-402

Beddall, Margaret; Chattopadhyay, Pratip K; Kao, Shing-Fen et al. (2016) A simple tube adapter to expedite and automate thawing of viably frozen cells. J Immunol Methods 439:74-78

Szomolay, Barbara; Liu, Jie; Brown, Paul E et al. (2016) Identification of human viral protein-derived ligands recognized by individual MHCI-restricted T-cell receptors. Immunol Cell Biol 94:573-82

Mahnke, Yolanda D; Beddall, Margaret H; Roederer, Mario (2015) OMIP-029: Human NK-cell phenotypization. Cytometry A :

Quinn, Kylie M; Zak, Daniel E; Costa, Andreia et al. (2015) Antigen expression determines adenoviral vaccine potency independent of IFN and STING signaling. J Clin Invest 125:1129-46

Pilipow, Karolina; Roberto, Alessandra; Roederer, Mario et al. (2015) IL15 and T-cell Stemness in T-cell-Based Cancer Immunotherapy. Cancer Res 75:5187-5193

Roederer, Mario (2015) A proposal for unified flow cytometer parameter naming. Cytometry A 87:689-91

Kibuuka, Hannah; Berkowitz, Nina M; Millard, Monica et al. (2015) Safety and immunogenicity of Ebola virus and Marburg virus glycoprotein DNA vaccines assessed separately and concomitantly in healthy Ugandan adults: a phase 1b, randomised, double-blind, placebo-controlled clinical trial. Lancet 385:1545-54