Abstract

We have immunopurified CENPA containing chromatin complexes from normal (HeLa) and 4 colorectal cancer cell lines. We find that there appear to be two fractions of CENPA in the genome and that CENPC may be differentially localized in these two fractions. Experiments are currently underway to precisely pinpoint using atomic force microscopy how CENPC is bound to CENPA. We have created multiple CENPA constructs with """"""""swapped"""""""" domains to further dissect precisely which part of the CENPA protein is essential for direct or indirect interaction with the CENPC and other core kinetochore proteins in human cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC011209-01
Application #
7966221
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2009
Total Cost
$211,846
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Quénet, Delphine; Dalal, Yamini (2015) Correction: a long non-coding RNA is required for targeting centromeric protein A to the human centromere. Elife 4:
Athwal, Rajbir K; Walkiewicz, Marcin P; Baek, Songjoon et al. (2015) CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells. Epigenetics Chromatin 8:2
Dalal, Yamini; McNally, James G (2014) Now you see it, now you don't: a biochemist's primer for advances in high-resolution microscopy: comment on ""Biomolecular dynamics and binding studies in the living cell"" by Stephan Diekmann and Christian Hoischen. Phys Life Rev 11:36-7; discussion 38
Volle, Catherine; Dalal, Yamini (2014) Histone variants: the tricksters of the chromatin world. Curr Opin Genet Dev 25:8-14,138
Bui, Minh; Walkiewicz, Marcin P; Dimitriadis, Emilios K et al. (2013) The CENP-A nucleosome: a battle between Dr Jekyll and Mr Hyde. Nucleus 4:37-42
Bui, Minh; Dimitriadis, Emilios K; Hoischen, Christian et al. (2012) Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo. Cell 150:317-26
Quénet, Delphine; McNally, James G; Dalal, Yamini (2012) Through thick and thin: the conundrum of chromatin fibre folding in vivo. EMBO Rep 13:943-4
Quénet, Delphine; Dalal, Yamini (2012) The CENP-A nucleosome: a dynamic structure and role at the centromere. Chromosome Res 20:465-79
Dalal, Yamini; Bui, Minh (2010) Down the rabbit hole of centromere assembly and dynamics. Curr Opin Cell Biol 22:392-402
Ambartsumyan, Gayane; Gill, Rajbir K; Perez, Silvia Diaz et al. (2010) Centromere protein A dynamics in human pluripotent stem cell self-renewal, differentiation and DNA damage. Hum Mol Genet 19:3970-82

Showing the most recent 10 out of 11 publications