Research in the Cellular Neurology Unit focuses on the molecular mechanisms underlying a number of neurodegenerative disorders, including mitochondrial disorders, dystonia, and the hereditary spastic paraplegias (HSPs). These disorders, which together afflict millions of Americans, worsen insidiously over a number of years, and treatment options are limited for many of them. Our laboratory is investigating inherited forms of these disorders, using molecular and cell biology approaches to study how mutations in disease genes ultimately result in cellular dysfunction. In this project, we are focusing on the HSPs. One major research theme involves the characterization and functional analysis of the hereditary spastic paraplegia type 3A (SPG3A) protein, atlastin-1. In 2009, we reported in the journal Cell that atlastin-1 is a member of a ubiquitous family of GTPases that interact with two families of ER shaping proteins to generate the tubular endoplasmic reticulum (ER) network. Interestingly, atlastin-1 interacts with the SPG31 protein REEP1, which is an ER shaping protein, as well as the SPG4 protein spastin, a microtubule-severing ATPase. In 2010, we published a study in the Journal of Clinical Investigation demonstrating that these three proteins interact with one another to organize the tubular ER network in conjunction with the microtubule cytoskeleton. Since SPG3A, SPG4, and SGP31 account for well over 50% of all HSP cases, we suggest ER network defects as the predominant neuropathologic mechanism for the HSPs. Over the past year, we have developed animal models for SPG31 (knock out) and SPG3A (knockout and knock in) to evaluate the extent of ER morphology changes using both in vivo and ex vivo studies. We are employing both electron microscopy and super-resolution confocal microscopy to examine the changes in tubular ER within neuronal axons in response to these genetic manipulations. In addition, we have identified interactions of these proteins with a protein mutated in another form of autosomal dominant HSP, expanding the number of HSP cases related to defects in ER network formation. Taken together, we expect that our studies will advance our understanding of the molecular pathogenesis of the HSPs. Such an understanding at the molecular and cellular levels will hopefully lead to novel treatments to prevent the progression of these disorders.
| Blackstone, Craig (2018) Hereditary spastic paraplegia. Handb Clin Neurol 148:633-652 |
| Blackstone, Craig (2018) Converging cellular themes for the hereditary spastic paraplegias. Curr Opin Neurobiol 51:139-146 |
| Zheng, Pengli; Chen, Qingzhou; Tian, Xiaoyu et al. (2018) DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Cell Res 28:833-854 |
| Guinto, Cheick O; Diarra, Salimata; Diallo, Salimata et al. (2017) A novel mutation in KIF5A in a Malian family with spastic paraplegia and sensory loss. Ann Clin Transl Neurol 4:272-275 |
| Roda, Ricardo H; Schindler, Alice B; Blackstone, Craig (2017) De novo REEP2 missense mutation in pure hereditary spastic paraplegia. Ann Clin Transl Neurol 4:347-350 |
| Blackstone, Craig (2017) Protein Targeting: ER Leads the Way to the Inner Nuclear Envelope. Curr Biol 27:R1284-R1286 |
| Blackstone, Craig (2017) A Larger BAT Improves Metabolism but Whiffs on Safety. EBioMedicine 24:9-10 |
| Byrne, Dwayne J; Harmon, Mark J; Simpson, Jeremy C et al. (2017) Roles for the VCP co-factors Npl4 and Ufd1 in neuronal function in Drosophila melanogaster. J Genet Genomics 44:493-501 |
| Nixon-Abell, Jonathon; Berwick, Daniel C; Grannó, Simone et al. (2016) Protective LRRK2 R1398H Variant Enhances GTPase and Wnt Signaling Activity. Front Mol Neurosci 9:18 |
| Renvoisé, Benoît; Malone, Brianna; Falgairolle, Melanie et al. (2016) Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation. Hum Mol Genet 25:5111-5125 |
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