Abstract

A variety of service and collaborative projects in protein identification have been or are being carried out within the Mass Spectrometry Research and Support Group with approximately 5000 samples analyzed from 33 scientists representing 21 principal investigators or core heads from 8 laboratory branches and the DNTP. One large effort is in support of the protein expression function of the Structural Biology Core Laboratory and Dr. Bob Petrovich. The Role of the MSRSG is to confirm gene expression at the protein level prior to the Structural Biology Core Laboratory handing materials over to their users. Other unpublished projects that are still ongoing include: Identification of bacterial pathogens by MALDI-MS David Kurtz Identification of binding partners of NTHL1 (Doetsch); Rubicon (Martinez); Grc3/Las1 (Stanley); TSEN (Stanley); SMCHD1 (Shaw); uncharacterized esterase (Williams); VRK1 (Negishi) Additional projects focused on the identification of proteins that interacted with RNA elements (Hall). Additional projects that have required more than negligible resources include efforts performed with the Blackshear, Fessler, Harry, R. London, and Hu laboratories.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICES102488-12
Application #
10012712
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Schellenberg, Matthew J; Lieberman, Jenna Ariel; Herrero-Ruiz, Andrés et al. (2017) ZATT (ZNF451)-mediated resolution of topoisomerase 2 DNA-protein cross-links. Science 357:1412-1416
Pillon, Monica C; Sobhany, Mack; Borgnia, Mario J et al. (2017) Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. Proc Natl Acad Sci U S A 114:E5530-E5538
Johnson, Katina L; Williams, Jason G; Maleki, Soheila J et al. (2016) Enhanced Approaches for Identifying Amadori Products: Application to Peanut Allergens. J Agric Food Chem 64:1406-13
Schellenberg, Matthew J; Perera, Lalith; Strom, Christina N et al. (2016) Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2. Nucleic Acids Res 44:3829-44
Yong, Sheila T; Nguyen, Hoai-Nghia; Choi, Jae H et al. (2015) Identification of a functional nuclear translocation sequence in hPPIP5K2. BMC Cell Biol 16:17
Prasad, Rajendra; Dyrkheeva, Nadezhda; Williams, Jason et al. (2015) Mammalian Base Excision Repair: Functional Partnership between PARP-1 and APE1 in AP-Site Repair. PLoS One 10:e0124269
Sharma, Nilesh K; Kumar, Ashutosh; Kumari, Amrita et al. (2015) Nitric Oxide Down-Regulates Topoisomerase I and Induces Camptothecin Resistance in Human Breast MCF-7 Tumor Cells. PLoS One 10:e0141897
ZeRuth, Gary T; Williams, Jason G; Cole, Yasemin C et al. (2015) HECT E3 Ubiquitin Ligase Itch Functions as a Novel Negative Regulator of Gli-Similar 3 (Glis3) Transcriptional Activity. PLoS One 10:e0131303
Martin, Negin P; Marron Fernandez de Velasco, Ezequiel; Mizuno, Fengxia et al. (2014) A rapid cytoplasmic mechanism for PI3 kinase regulation by the nuclear thyroid hormone receptor, TR?, and genetic evidence for its role in the maturation of mouse hippocampal synapses in vivo. Endocrinology 155:3713-24
Horton, Julie K; Stefanick, Donna F; Gassman, Natalie R et al. (2013) Preventing oxidation of cellular XRCC1 affects PARP-mediated DNA damage responses. DNA Repair (Amst) 12:774-85

Showing the most recent 10 out of 29 publications