Biophysics Core Facility

Piszczek, Grzegorz

Abstract

The services that the Biophysics Core Facility provides to the NIH scientific community consist of four main categories: direct research support including instrument use, collaborations on research projects and scientific consultations, development and optimization of measurement methods, and user training. In the reporting period, the total workload of the Biophysics Core Facility exceeded 4,000 instrument/hours. During this time, the Biophysics Core Facility users included principal investigators, staff scientists, postdocs and students from the following labs (53 laboratories): NIH laboratories (listed by the PIs last name): Adhya, Sankar (NCI); Altan-Bonnet, Nihal (NHLBI); Balaban, Robert (NHLBI); Bernstein, Harris (NIDDK); Bonifacino, Juan (NICHD); Buchanan, Susan (NIDDK); Chattoraj, Dhruba K. (NCI); Chiorini, Jay (NIDCR); Cookson, Mark (NIA); Dyda, Frederick (NIDDK); Escorcia, Freddy (NCI); Farber, Joshua (NIAID); Ferre-DAmare, Adrian (NHLBI); Fischbeck, Kenneth (NINDS); Fitzgerald, David (NCI); Hinton, Deborah (NIDDK); Ho, Mitchell (NCI); Hogg, Bobby (NHLBI); Jiang, Jiansen (NHLBI); Jin, Tian (NIAID); Kemper, Claudia (NHLBI); Khan, Javed (NCI); Koretsky, Alan (NINDS); Korn, Edward (NHLBI); Kovac, Paul (NIDDK); Larochelle, Andre (NHLBI); Lee, Jennifer (NHLBI); Lee, Kyung (NCI-NHLBI); Levine, Rodney (NHLBI); Loh, Yoke Peng (NICHD); Mizuuchi, Kiyoshi (NIDDK); Narendra, Derek (NINDS); Neuman, Keir (NHLBI); Otto, Michael (NIAID); Peterkofsky, Alan (NHLBI); Philpott, Caroline (NIDDK); Ramamurthi, Kumaran (NCI); Redmond, Thomas Michael (NEI); Remaley, Alan (NHLBI); Roberts, David (NCI); Roll-Mecak, Antonina (NHLBI); Rusan, Nasser (NHLBI); Sellers, James (NHLBI); Simeonov, Anton (NCATS); Steeg, Patricia S. (NCI); Tabak, Lawrence (NIDCR); Tanner, Kandice (NCI); Tjandra, Nico (NHLBI); Wickner, Sue (NCI); Wistow, Graeme (NEI); Wu, Chuan (non-NHLBI); Zhang, Jinwei (NIDDK); Zhuang, Zhengping (NCI);

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Scientific Cores Intramural Research (ZIC)
• Project #: 1ZICHL000338-19
• Application #: 10023088
• Support Year: 19
• Fiscal Year: 2019

Institution

• Name: National Heart, Lung, and Blood Institute

Publications

Liu, Xiong; Billington, Neil; Shu, Shi et al. (2017) Effect of ATP and regulatory light-chain phosphorylation on the polymerization of mammalian nonmuscle myosin II. Proc Natl Acad Sci U S A 114:E6516-E6525

O'Neil, Patrick K; Richardson, Lynn G L; Paila, Yamuna D et al. (2017) The POTRA domains of Toc75 exhibit chaperone-like function to facilitate import into chloroplasts. Proc Natl Acad Sci U S A 114:E4868-E4876

Hayoz, Sebastien; Tiwari, Purushottam B; Piszczek, Grzegorz et al. (2017) Investigating cyclic nucleotide and cyclic dinucleotide binding to HCN channels by surface plasmon resonance. PLoS One 12:e0185359

Jung, Goeh; Alexander, Christopher J; Wu, Xufeng S et al. (2016) V-1 regulates capping protein activity in vivo. Proc Natl Acad Sci U S A 113:E6610-E6619

Liu, W; Li, H; Hong, S-H et al. (2016) Olfactomedin 4 deletion induces colon adenocarcinoma in Apc(Min/+) mice. Oncogene :

Paez-Segala, Maria G; Sun, Mei G; Shtengel, Gleb et al. (2015) Fixation-resistant photoactivatable fluorescent proteins for CLEM. Nat Methods 12:215-8, 4 p following 218

Zhao, Huaying; Piszczek, Grzegorz; Schuck, Peter (2015) SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions. Methods 76:137-148

Zhao, Huaying; Ghirlando, Rodolfo; Alfonso, Carlos et al. (2015) A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS One 10:e0126420

Gruschus, James M; Jiang, Zhiping; Yap, Thai Leong et al. (2015) Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C. Biochem Biophys Res Commun 457:561-6

Zhao, Huaying; Ma, Jia; Ingaramo, Maria et al. (2014) Accounting for photophysical processes and specific signal intensity changes in fluorescence-detected sedimentation velocity. Anal Chem 86:9286-92