This proposal was received in response to Nanoscale Science and Engineering initiative, NSF 02-148, category NIRT. The research focuses on the characterization and application of S-layer proteins, which are molecularly thin protein sheet crystals located on the outer cell envelope of a variety of micro-organisms. The crystal lattices incorporate nanoscopic pores located on repetitive lattice sites and can function as natural ultrafiltration devices. Reinforced S-layer lipid membrane hybrid architectures will be implemented on mesoporous, macroscopic substrates leading to functional, macroscopically-stabilized biomembrane mimics, accessible from both sides by small molecular species. Nanoporous gold will provide the interface to the macroscopic world. As a model of biomembrane functionalization, hemolysin will be assembled into the S-layer for use in measuring the passage of a single molecule. With these structures it may be possible to thread single-stranded DNA through the pores and to attach proteins to the ends of the DNA, thus engineering the capture and release of DNA from the pore. In terms of the broader impacts, these structures may find applications in a variety of devices including membranes and sensors. Collaborations with NIST and a transatlantic educational program are planned.

Project Start
Project End
Budget Start
2003-09-01
Budget End
2006-02-28
Support Year
Fiscal Year
2003
Total Cost
$1,175,301
Indirect Cost
Name
Johns Hopkins University
Department
Type
DUNS #
City
Baltimore
State
MD
Country
United States
Zip Code
21218