This research effort is aimed at understanding the basic metabolism of microbial and mammalian cells and to use that information to alter the productivity of the reaction system. A liquid scintillation counter will be used for the following projects: (1) effects of biopolymer particulates on the metabolism and ecology of bacterial biofilms; (2) enhanced enzyme productivity in Hansenula polymorpha by substrate composition and growth rate transients; (3) influence of adhesion on the metabolism of microbes genetically-engineered for xenobiotic compound degradation in the ground water; (4) thermochemical control of secondary metabolite quality, yield, and production rate-synthesis of the polysaccharide Xanthan by Xanthamonas campestris; and (5) engineering studies of kinetics and optimization of monoclonal antibody production by a hybridoma.
