The objective of this project is to develop soluble complexes of DNA with cationic polymers for use in gene delivery to cells. The objectives described in the proposal are threefold: (1) to examine the efficacy of gene transfer of DNA/polycation complexes of various molecular architectures; (2) to study cellular mechanisms for delivery of the complexes into the cell; and (3) to evaluate the efficacy of gene transfer of DNA/polycation complexes containing attached proteins and peptides.
