The pathogenic bacterium Ralstonia solanacearum is very successful in the vascular tissue of its plant hosts even though this habitat contains few nutrients and little oxygen. This project's central hypothesis is that R. solanacearum uses inorganic nitrogen, one of the few abundant nutrients in plant sap, not just for food but also to generate energy and to sense and manipulate plant defenses. The investigators will use genetic and biochemical experiments to identify the specific adaptations that allow this pathogen to use inorganic nitrogen in multiple ways as it causes plant disease. Expected results include a better understanding of how R. solanacearum grows at low oxygen levels and a definition of the role(s) in virulence of nitric oxide, a common biological signal molecule that also has antimicrobial properties. R. solanacearum is a widely distributed pathogen with many hosts, so these results are likely to be relevant to diverse plant-associated microbes. Plant vascular systems are home to many microbes, ranging from destructive pathogens like R. solanacearum to valuable mutualists that can increase plant stress tolerance or growth.

This project will generate basic biological knowledge about the biochemical mechanisms used by plant-inhabiting microbes. In addition, this project will contribute to the nation's scientific workforce by training a postdoctoral researcher and a doctoral student in laboratory research, teaching and mentoring, which are all key skills for future faculty. Three undergraduate students will also receive independent research experiences in microbiology.

Agency
National Science Foundation (NSF)
Institute
Division of Integrative Organismal Systems (IOS)
Application #
1258082
Program Officer
Michael Mishkind
Project Start
Project End
Budget Start
2013-03-15
Budget End
2017-02-28
Support Year
Fiscal Year
2012
Total Cost
$508,000
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
City
Madison
State
WI
Country
United States
Zip Code
53715