9310850 Roberts The rates of malate metabolism and transport in maize root tips will be measured using nuclear magnetic resonance spectroscopy in combination with other biochemical techniques. These processes will be quantitated in tissue exhibiting different rates of malate synthesis, in response to potassium ion uptake, or assimilation of ammonium ion. Specific reactions that will be monitored include (i) utilization of glycolytically-derived malate by mitochondria for oxidation; (ii) action of PEP carboxylase and malic enzyme, and their coordination with activity in the Krebs cycle and amino acid biosynthesis; (iii) movement of malate between cytoplasm and vacuole. The combined measurements will permit observation of interactions between the different components comprising malate metabolism. This information is a prerequisite to an understanding of respiration, mineral nutrition, intracellular pH regulation, amino acid biosynthesis and growth - particularly with respect to the integration of these processes in vivo. ***

Agency
National Science Foundation (NSF)
Institute
Division of Integrative Organismal Systems (IOS)
Application #
9310850
Program Officer
Erik T. Nilsen
Project Start
Project End
Budget Start
1993-08-15
Budget End
1997-09-30
Support Year
Fiscal Year
1993
Total Cost
$230,000
Indirect Cost
Name
University of California Riverside
Department
Type
DUNS #
City
Riverside
State
CA
Country
United States
Zip Code
92521