The long-term goals of the proposed research project are to understand the molecular basis of and regulation of sequence specific protein-DNA recognition reactions leading to transposition. This will be pursued by studying the IS50/Tn5 transposition system. Transposable elements, such as IS50/Tn5, are genetic entities which can catalyze numerous types of genome rearrangements. They are thought to have been important in evolution and can lead to somatic cell mutations such as those associated with oncogenesis. Genetic and biochemical approaches will be used to analyze the interaction of IS50/Tn5 transposase with its target DNA sequences, the regulation of transposase activity by its inhibitor and the role of host proteins in the transposition process.

Agency
National Science Foundation (NSF)
Institute
Division of Molecular and Cellular Biosciences (MCB)
Application #
9020517
Program Officer
DeLill Nasser
Project Start
Project End
Budget Start
1991-02-01
Budget End
1994-01-31
Support Year
Fiscal Year
1990
Total Cost
$310,000
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
City
Madison
State
WI
Country
United States
Zip Code
53715