The central goal of this project will be to develop active interfaces between arrays of addressable nanowire electronic devices and cells to create two- and three-dimensional functional biomaterials. The nanoscale represents a natural length scale for devices used to create active interfaces to cells because biological nanostructures are the key components that define intercellular communication and signaling in living systems, yet this scale of interface is virtually uncharted research territory. The overall objectives that will be explored to address this territory and to meet the central project goal are as follows. First, limits of nanowire nanoelectronic device/cell interface functionality and sensitivity will be characterized. Communication with cells, both recording from (cell output) and stimulation of (cell input), through direct electrical mechanisms and chemical/biological signal detection/release will be developed and the corresponding detection sensitivity and spatial resolution for different modes will be elucidated. Second, addressable arrays of different types of functional nanowire devices interfaced with cellular networks will be developed and their behavior delineated. An important emphasis of this work, which exploits the unique capabilities of bottom-up versus top-down nanoscience, will be to develop (i) interfaced nanoelectronic arrays on flexible, biocompatible polymeric substrates, (ii) folded or layered nanowire/cell three-dimensional arrays using perforated polymeric substrates to enhance layer-to-layer interactions, and (iii) nanoelectronic devices projecting out of the substrate plane to enable interfacing `into' three-dimensional cellular arrays and tissue. The proposed project has the potential to impact biomedical research in substantial ways, including the establishment of powerful new tools for understanding the behavior of interacting cellular networks, the development of sophisticated, electrically-based cell/tissue interfaces for prosthetics and other medical devices, and the creation of new biomaterials with potential for diverse applications, such as in hybrid information processing.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
NIH Director’s Pioneer Award (NDPA) (DP1)
Project #
5DP1OD003900-04
Application #
8119451
Study Section
Special Emphasis Panel (ZGM1-NDPA-B (P2))
Program Officer
Wehrle, Janna P
Project Start
2008-09-30
Project End
2012-07-31
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
4
Fiscal Year
2011
Total Cost
$831,600
Indirect Cost
Name
Harvard University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
082359691
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Qing, Quan; Jiang, Zhe; Xu, Lin et al. (2014) Free-standing kinked nanowire transistor probes for targeted intracellular recording in three dimensions. Nat Nanotechnol 9:142-7
Duan, Xiaojie; Lieber, Charles M (2013) Nanoelectronics meets biology: from new nanoscale devices for live-cell recording to 3D innervated tissues. Chem Asian J 8:2304-14
Duan, Xiaojie; Fu, Tian-Ming; Liu, Jia et al. (2013) Nanoelectronics-biology frontier: From nanoscopic probes for action potential recording in live cells to three-dimensional cyborg tissues. Nano Today 8:351-373
Tian, Bozhi; Lieber, Charles M (2013) Synthetic nanoelectronic probes for biological cells and tissues. Annu Rev Anal Chem (Palo Alto Calif) 6:31-51
Xu, Lin; Jiang, Zhe; Qing, Quan et al. (2013) Design and synthesis of diverse functional kinked nanowire structures for nanoelectronic bioprobes. Nano Lett 13:746-51
Duan, Xiaojie; Gao, Ruixuan; Xie, Ping et al. (2012) Intracellular recordings of action potentials by an extracellular nanoscale field-effect transistor. Nat Nanotechnol 7:174-9
Xie, Ping; Xiong, Qihua; Fang, Ying et al. (2012) Local electrical potential detection of DNA by nanowire-nanopore sensors. Nat Nanotechnol 7:119-25
Jiang, Zhe; Qing, Quan; Xie, Ping et al. (2012) Kinked p-n junction nanowire probes for high spatial resolution sensing and intracellular recording. Nano Lett 12:1711-6
Park, Jang-Ung; Nam, SungWoo; Lee, Mi-Sun et al. (2012) Synthesis of monolithic graphene-graphite integrated electronics. Nat Mater 11:120-5
Tian, Bozhi; Liu, Jia; Dvir, Tal et al. (2012) Macroporous nanowire nanoelectronic scaffolds for synthetic tissues. Nat Mater 11:986-94

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