The objective of this project is to characterize and clarify, from cellular to molecular level, the mechanism of activation of phospholipase A2 in a rat mast cell line, rat basophilic leukemia (RBL-2H3) cells. Therefore, my main goals are: 1. To determine whether release of arachidonic acid in RBL-2H3 cells is dependent on activation of existing phospholipase A2 or expression of new enzyme. 2. To identify isozymes of phospholipase A2 in RBL-2H3 cells and to prepare the antibodies to them. 3. To determine whether phospholipase A2 can be activated via G- proteins and/or tyrosine phosphorylation.

Agency
National Institute of Health (NIH)
Institute
Fogarty International Center (FIC)
Type
International Research Fellowships (FIC) (F05)
Project #
3F05TW004850-02S2
Application #
2292971
Study Section
International and Cooperative Projects 1 Study Section (ICP)
Project Start
1994-09-30
Project End
Budget Start
1994-05-01
Budget End
1994-10-31
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost
Name
U.S. National Heart Lung and Blood Institute
Department
Type
DUNS #
City
Bethesda
State
MD
Country
United States
Zip Code
20892