Implanted biomaterials are finding increasing use in both dental and orthopedic applications in an aging American population. To enhance the incorporation of materials into the body, it is important to elucidate the factors that influence successful tissue-biomaterial interactions. Of particular interest is the role of micron-scale surface topography in determining the success of biomaterials. The objective of this investigation is to examine the effects of novel surface topographies on an osteoblastic-like cell line in vitro. The hypothesis to be tested in this study states that precise topographical features can modulate the attachment, morphology, proliferation, differentiation, and protein production of HOS TE85 cells in vitro in a predictable manner. The hypothesis is to be addressed by the following specific aims: (1) Create a family of polystyrene substrates with controlled, engineered surface topographies fabricated by Micro-Electro-Mechanical Systems (MEMS) technology. (2) Examine modulation of attachment, cell morphology, proliferation, differentiation and protein production with engineered surface topographies. (3) Investigate the role of substrate chemistry and composition on cellular attachment, proliferation, and differentiation. Techniques to be utilized include maintaining bone cells in culture, solvent casting of polystyrene substrates, light and scanning electron microscopy, and immunohistochemistry.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Individual Predoctoral NRSA for M.D./Ph.D. Fellowships (ADAMHA) (F30)
Project #
3F30DE014658-02S1
Application #
6895987
Study Section
NIDCR Special Grants Review Committee (DSR)
Program Officer
Hardwick, Kevin S
Project Start
2002-08-05
Project End
2004-08-04
Budget Start
2003-08-05
Budget End
2004-08-04
Support Year
2
Fiscal Year
2004
Total Cost
$800
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Dentistry
Type
Schools of Dentistry
DUNS #
623946217
City
Newark
State
NJ
Country
United States
Zip Code
07107