The specific aims of this project are to expand the understanding of how chronic ethanol exposure affects the release of hormones and ion channel electrophysiology in the intact neurohypophysis and isolated neurohypophysial terminals. Ethanol has been known to cause diuresis and acute applications to isolated neurohypophysial terminals have been shown to inhibit Ca+2 current, potentiate CAK current, and suppress vasopressin release. This, coupled with the experimental accessibility of the neurohypophysis, makes it an ideal system to test for chronic ethanol effects. The problem will be studied using a combined biochemical and electrophysiological strategy. Chronic ethanol-induced changes in cellular electrical activity will be studied using whole cell perforated patch clamp techniques, while stimulus-release coupling and radioimmunoassays will be used to study hormone release. The long range goal is to utilize this knowledge for the development of better treatments for ethanol addiction.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31AA005524-03
Application #
6168190
Study Section
Special Emphasis Panel (ZAA1-DD (01))
Program Officer
Hunt, Walter
Project Start
2000-09-08
Project End
Budget Start
2000-09-08
Budget End
2001-01-22
Support Year
3
Fiscal Year
2000
Total Cost
$9,364
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Pharmacology
Type
Schools of Medicine
DUNS #
660735098
City
Worcester
State
MA
Country
United States
Zip Code
01655
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