R-Ras is a small GTPase related to the Ras oncogene. How R-Ras exerts its biological effects in regulation of cell growth, adhesion, survival and oncogenesis is not clear, as few effectors have been identified to date. One possible mediator of R-Ras function is the novel phospholipase C protein, PLCepsilon (PLCe). R-Ras binds and activates the phospholipase activity of PLCe in vitro. Whether this activation is biologically relevant to R-Ras signaling, transformation, cytoskeletal regulation and survival is examined in the studies proposed here. If the studies are successful, the results may help explain how oncogenic R-Ras contributes to the aberrant phenotype of a cancer cell.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31CA103143-02
Application #
6780391
Study Section
Special Emphasis Panel (ZRG1-F05 (29))
Program Officer
Bini, Alessandra M
Project Start
2003-07-15
Project End
2007-07-14
Budget Start
2004-07-15
Budget End
2005-07-14
Support Year
2
Fiscal Year
2004
Total Cost
$26,899
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Alan, Jamie K; Berzat, Anastacia C; Dewar, Brian J et al. (2010) Regulation of the Rho family small GTPase Wrch-1/RhoU by C-terminal tyrosine phosphorylation requires Src. Mol Cell Biol 30:4324-38
Berzat, Anastacia C; Buss, Janice E; Chenette, Emily J et al. (2005) Transforming activity of the Rho family GTPase, Wrch-1, a Wnt-regulated Cdc42 homolog, is dependent on a novel carboxyl-terminal palmitoylation motif. J Biol Chem 280:33055-65