My goal is to elucidate the role of the Androgen Receptor Trapped clone-27 (ART-27) in androgen mediated prostate cell growth and differentiation. ART-27 is a newly described transcriptional co-activator of the androgen receptor (AR) and may play a role as a tumor suppressor gene in the prostate. I hypothesize that ART-27 facilitates androgen-dependent differentiation of prostate epithelial cells by helping AR regulate a subset of genes important to prostate growth suppression and differentiation. I further hypothesize that alterations in the level of ART-27 modulates AR activity, which, in turn, affect AR-dependent cell growth regulation in vivo. To test these hypotheses, I will 1) analyze AR-dependent gene expression as a function of ART-27 in prostate cancer cells using DNA microarrays; 2) assess the effect(s) of ART-27 overexpression in prostatic epithelia in vivo using a transgenic mouse model; 3) Identify factors that interact with ART-27 using the yeast two hybrid system.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31CA113285-04
Application #
7328604
Study Section
Special Emphasis Panel (ZRG1-IDM-L (29))
Program Officer
Bini, Alessandra M
Project Start
2004-12-01
Project End
2008-11-30
Budget Start
2007-12-01
Budget End
2008-11-30
Support Year
4
Fiscal Year
2008
Total Cost
$46,864
Indirect Cost
Name
New York University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Mita, Paolo; Savas, Jeffrey N; Ha, Susan et al. (2013) Analysis of URI nuclear interaction with RPB5 and components of the R2TP/prefoldin-like complex. PLoS One 8:e63879
Mita, Paolo; Savas, Jeffrey N; Djouder, Nabil et al. (2011) Regulation of androgen receptor-mediated transcription by RPB5 binding protein URI/RMP. Mol Cell Biol 31:3639-52
Nwachukwu, Jerome C; Mita, Paolo; Ruoff, Rachel et al. (2009) Genome-wide impact of androgen receptor trapped clone-27 loss on androgen-regulated transcription in prostate cancer cells. Cancer Res 69:3140-7
Nwachukwu, Jerome C; Li, Wenhui; Pineda-Torra, Ines et al. (2007) Transcriptional regulation of the androgen receptor cofactor androgen receptor trapped clone-27. Mol Endocrinol 21:2864-76