Abstract

The proposed research examines the influence of hydrodynamic cell-cell and cell-vessel wall interactions under physiological flow conditions on platelet adhesion dynamics at microscale resolution.
The specific aims of this proposal are: 1. 3-D modeling of platelet adhesion with reactive surfaces under flow, and 2. Experimental and theoretical characterization of cell aggregation under flow. A recently developed rigorous numerical simulation called Multiparticle Adhesive Dynamics (MAD) models the adhesion of spherical cells to surfaces in any general flow field, and will be extended to simulate 3-D platelet (oblate spheroid) adhesive interactions with surface-bound von Willebrand factor in shear flow. The simulation will include cell-cell (RBC-platelet) hydrodynamic interactions as well as cell aggregation (neutrophil-platelet) separately. Flow experiments of platelet adhesion in solutions of varying hematocrit will be conducted to determine the influence of RBC-platelet collisions on platelet deposition rate. Cell aggregation between platelets and leukocytes near a surface in shear field will also be examined experimentally.
This research aims to characterize the interplay between specific chemical adhesion and hydrodynamic forces.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
1F31EB005104-01
Application #
6935127
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Khachaturian, Henry
Project Start
2005-03-16
Project End
2007-03-15
Budget Start
2005-03-16
Budget End
2006-03-15
Support Year
1
Fiscal Year
2005
Total Cost
$24,952
Indirect Cost

  Institution

Name
University of Rochester
Department
Biomedical Engineering
Type
Schools of Dentistry
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627