The hypothesis of this proposal is that optical coherence microscopy (OCM), a combination of OCT with confocal microscopy, can enable cellular resolution endoscopic imaging of tissue morphology, thereby dramatically advancing the capabilities of either technique for endoscopic detection of early stage neoplasms. An endoscopic """"""""optical biopsy"""""""" technique that allows for real time, in vivo imaging of pre- cancerous changes at the cellular level would allow image guidance of traditional excisional biopsy, potentially reducing sampling error and improving sensitivity.
The specific aims are: 1) Develop optical coherence microscopy (OCM) technology for cellular imaging of unstained tissues. 2) Investigate OCM for detecting early neoplastic cellular alterations in human tissues through ex vivo clinical imaging studies. 3) Develop miniaturized flexible endoscopes for OCM imaging based on micro-electro-mechanical systems (MEMS) scanning technology. 4) Validate flexible OCM endoscopes through in vivo animal imaging experiments. These studies combined seek to demonstrate fundamental new advances for endoscopic detection of early stage neoplasms.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31EB005978-02
Application #
7173337
Study Section
Special Emphasis Panel (ZRG1-GGG-G (29))
Program Officer
Erim, Zeynep
Project Start
2006-01-16
Project End
2008-04-15
Budget Start
2007-01-16
Budget End
2008-01-15
Support Year
2
Fiscal Year
2007
Total Cost
$44,282
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Organized Research Units
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Jia, Haibo; Abtahian, Farhad; Aguirre, Aaron D et al. (2013) In vivo diagnosis of plaque erosion and calcified nodule in patients with acute coronary syndrome by intravascular optical coherence tomography. J Am Coll Cardiol 62:1748-58
Aguirre, Aaron D; Chen, Yu; Bryan, Bradley et al. (2010) Cellular resolution ex vivo imaging of gastrointestinal tissues with optical coherence microscopy. J Biomed Opt 15:016025
Aguirre, Aaron D; Sawinski, Juergen; Huang, Shu-Wei et al. (2010) High speed optical coherence microscopy with autofocus adjustment and a miniaturized endoscopic imaging probe. Opt Express 18:4222-39
Chen, Yu; Aguirre, Aaron D; Ruvinskaya, Lana et al. (2009) Optical coherence tomography (OCT) reveals depth-resolved dynamics during functional brain activation. J Neurosci Methods 178:162-73
Chen, Yu; Huang, Shu-Wei; Aguirre, Aaron D et al. (2007) High-resolution line-scanning optical coherence microscopy. Opt Lett 32:1971-3
Aguirre, A D; Chen, Y; Fujimoto, J G et al. (2006) Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography. Opt Lett 31:3459-61