We will investigate the formation of DNA C-1' radicals that are produced by laser flash photolysis (LFP) and determine the UV spectra of the radicals and kinetics of reactions with various trapping agents, such as 02 and thiols. The DNA C-1' radical precursors will be synthesized from known intermediates. The successful generation of radicals in the DNA C-1' position could provide additional tools to be used in the study of radical induced DNA damage. The DNA C-1' radical has been shown to be a common intermediate in DNA damage. Neurostatin and bis- phenanthroline complex of copper generate a DNA C-1' radical which can lead to DNA damage by strand cleavage. When trapped by thiols, the DNA C-1' radical can lead to the premutagenic alpha anomer of the nucleoside.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31ES005833-02
Application #
2909982
Study Section
Special Emphasis Panel (ZRG2-SSS-F (06))
Program Officer
Gorelic, Lester S
Project Start
1999-05-01
Project End
Budget Start
1999-05-01
Budget End
2000-04-30
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Wayne State University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202